Human/Mouse Olig3 Antibody

Catalog #: MAB24561 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB24561-100
MAB24561-SP

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Human/Mouse Olig3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Olig3 in direct ELISAs. Detects mouse Olig3 in Immunohistochemistry. In direct ELISA, no cross-reactivity with recombinant human (rh) Olig1 or rhOlig2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 257914

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Olig3
Met1-Lys272
Accession # Q7RTU3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Olig3 in 13 dpc Mouse Embryo Tissue. Olig3 was detected in immersion fixed frozen sections of 13 dpc mouse embryo tissue using Mouse Anti-Human/Mouse Olig3 Monoclonal Antibody (Catalog # MAB24561) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig3

Olig3 belongs to the Olig family of basic-helix-loop-helix (bHLH) transcription factors. It is transiently expressed in different types of embryonic central nervous system progenitors but is absent in later stages of development.

Long Name

Oligodendrocyte Lineage Transcription Factor 3

Entrez Gene IDs

167826 (Human); 94222 (Mouse)

Alternate Names

BHLHB7; BHLHE20; bHLHe20oligo3; Class B basic helix-loop-helix protein 7; Class E basic helix-loop-helix protein 20; Olig3; Oligo3; oligodendrocyte transcription factor 3