Human/Mouse Olig3 Antibody

Catalog # Availability Size / Price Qty
MAB2456
MAB2456-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human/Mouse Olig3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Olig3 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Olig1 or rhOlig2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 257934
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Olig3
Met1-Lys272
Accession # Q7RTU3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Olig3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig3

Olig3 belongs to the Olig family of basic-helix-loop-helix (bHLH) transcription factors. It is transiently expressed in different types of embryonic central nervous system progenitors but is absent in later stages of development.

Long Name
Oligodendrocyte Lineage Transcription Factor 3
Entrez Gene IDs
167826 (Human); 94222 (Mouse)
Alternate Names
BHLHB7; BHLHE20; bHLHe20oligo3; Class B basic helix-loop-helix protein 7; Class E basic helix-loop-helix protein 20; Olig3; Oligo3; oligodendrocyte transcription factor 3

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Citations for Human/Mouse Olig3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants
    Authors: B Sanders, D D'Andrea, MO Collins, E Rees, TGJ Steward, Y Zhu, G Chapman, SE Legge, AF Pardiñas, AJ Harwood, WP Gray, MC O'Donovan, MJ Owen, AC Errington, DJ Blake, DJ Whitcomb, AJ Pocklingto, E Shin
    Nature Communications, 2022-01-14;13(1):27.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Ontogenetic establishment of order-specific nuclear organization in the mammalian thalamus
    Authors: W Shi, A Xianyu, Z Han, X Tang, Z Li, H Zhong, T Mao, K Huang, SH Shi
    Nat. Neurosci, 2017-02-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Generation of thalamic neurons from mouse embryonic stem cells
    Authors: A Shiraishi, K Muguruma, Y Sasai
    Development, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  4. Characterization of progenitor domains in the developing mouse thalamus.
    Authors: Vue TY, Aaker J, Taniguchi A
    J. Comp. Neurol., 2007-11-01;505(1):73-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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