Human/Mouse Otoraplin/OTOR Antibody

Catalog #: AF3979 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3979
AF3979-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Otoraplin/OTOR Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Otoraplin/OTOR in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Otoraplin/OTOR
Val18-Glu128
Accession # Q9NRC9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human and Mouse Otoraplin/OTOR

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Otoraplin/OTOR

Otoraplin (OTOR), also known as fibrocyte-derived protein (Fdp) and Melanoma inhibitory activity-like (MIAL), is a secreted 16 kDa globular protein that is preferentially expressed in the inner ear by periotic mesenchyme and developing and mature fibrocytes. It is highly homologous to MIA/cartilage-derived retinoic acid‑sensitive protein (CD-RAP), which is a cartilage-specific protein that is also expressed in malignant melanoma cells. The 111 amino acid residue (aa) mature human otoraplin contains one SH3 domain (aa 46‑107) and a Tyr at position 50 that is reportedly sulfated. Otoraplin is reported to play a role in the initiation of periotic mesenchyme chondrogenesis. Mature human Otoraplin shares 91% and 89% aa sequence identity with canine and mouse Otoraplin, respectively.

Entrez Gene IDs

56914 (Human); 57329 (Mouse); 366206 (Rat)

Alternate Names

FDPMelanoma inhibitory activity-like protein; Fibrocyte-derived protein; MGC126739; MIAL1; MIALMGC126737; OTOR; Otoraplin