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Human/Mouse PDX-1/IPF1 Antibody

Catalog #: MAB2419
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2419
MAB2419-SP
Detection of Mouse PDX‑1/IPF1 by Western Blot.
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Product Details
Citations (12)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PDX-1/IPF1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse PDX-1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 267712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PDX-1
Ala91-Arg283
Accession # P52945
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
beta TC-6 mouse beta cell insulinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse PDX-1/IPF1 antibody by Western Blot. View Larger

Detection of Mouse PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX-1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PDX-1/IPF1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX-1/IPF1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry PDX-1/IPF1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Intracellular Staining by Flow Cytometry View Larger

Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Immunohistochemistry View Larger

Detection of PDX‑1/IPF1 in Human Pancreas. PDX‑1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus in islet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDX-1/IPF1

Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.

Long Name
Pancreas/Duodenum Homeobox-1
Entrez Gene IDs
3651 (Human); 18609 (Mouse)
Alternate Names
Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1

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Citations for Human/Mouse PDX-1/IPF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. YAP1 and TAZ Control Pancreatic Cancer Initiation in Mice by Direct Up-regulation of JAK–STAT3 Signaling
    Authors: Ralph Gruber, Richard Panayiotou, Emma Nye, Bradley Spencer-Dene, Gordon Stamp, Axel Behrens
    Gastroenterology
  2. DNA methylation reveals distinct cells of origin for pancreatic neuroendocrine carcinomas and pancreatic neuroendocrine tumors
    Authors: Tincy Simon, Pamela Riemer, Armin Jarosch, Katharina Detjen, Annunziata Di Domenico, Felix Bormann et al.
    Genome Medicine
  3. Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity
    Authors: ET Usher, N Sabri, R Rohac, AK Boal, T Mittag, SA Showalter
    The Journal of Biological Chemistry, 2021-04-22;0(0):100693.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells
    Authors: P Saxena, BC Heng, P Bai, M Folcher, H Zulewski, M Fussenegge
    Nat Commun, 2016-04-11;7(0):11247.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. Redifferentiation of adult human beta cells expanded in vitro by inhibition of the WNT pathway.
    Authors: Lenz A, Toren-Haritan G, Efrat S
    PLoS ONE, 2014-11-13;9(11):e112914.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Peroxisome proliferator-activated receptor gamma activation restores islet function in diabetic mice through reduction of endoplasmic reticulum stress and maintenance of euchromatin structure.
    Authors: Evans-Molina C, Robbins RD, Kono T, Tersey SA, Vestermark GL, Nunemaker CS, Garmey JC, Deering TG, Keller SR, Maier B, Mirmira RG
    Mol. Cell. Biol., 2009-02-23;29(8):2053-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells.
    Authors: Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK
    Biochem. Biophys. Res. Commun., 2007-12-04;366(1):129-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells.
    Authors: Li Y, Zhang R, Qiao H, Zhang H, Wang Y, Yuan H, Liu Q, Liu D, Chen L, Pei X
    J. Cell. Physiol., 2007-04-01;211(1):36-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Epigenetic landscape of pancreatic neuroendocrine tumours reveals distinct cells of origin and means of tumour progression
    Authors: Annunziata Di Domenico, Christodoulos P. Pipinikas, Renaud S. Maire, Konstantin Bräutigam, Cedric Simillion, Matthias S. Dettmer et al.
    Communications Biology
  10. The combined effect of PDX1, epidermal growth factor and poly-L-ornithine on human amnion epithelial cells’ differentiation
    Authors: Shruti Balaji, Yu Zhou, Anasuya Ganguly, Emmanuel C. Opara, Shay Soker
    BMC Developmental Biology
  11. Coxsackievirus B Type 4 Infection in beta Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing
    Authors: Bernard H, Teijeiro A, Chaves-Perez A et al.
    Cell Reports Medicine
  12. Pdx1 Expression in Pancreatic Precursor Lesions and Neoplasms
    Authors: Jason Y. Park, Seung-Mo Hong, David S. Klimstra, Michael G. Goggins, Anirban Maitra, Ralph H. Hruban
    Applied Immunohistochemistry & Molecular Morphology

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Human/Mouse PDX-1/IPF1 Antibody
By Anonymous on 02/11/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: BG01V human embryonic stem cells Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse PDX-1/IPF1 Antibody MAB2419