Human/Mouse PDX-1/IPF1 APC-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: PDX-1/IPF1

PDX-1 (Pancreatic-duodenal Homeobox Factor-1), also known as IDX-1 and STF-1 is a 31 kDa member of the IPF-1 (Insulin Promoter Factor) subfamily, Antp (antennapedia) family of molecules. Human PDX-1 is 283 amino acids (aa) in length, and although its predicted MW is approximately 30 kDa, it runs anomalously in SDS-PAGE at 40-46 kDa. PDX-1 possesses one transctivation domain (aa 13-73) and one DNA-binding homeodomain (aa 146-205) that contains an NLS between aa 197-203. Phosphorylation is known to occur on Ser61 and Ser269, and methylation is found on Lys131. PDX-1 drives the development of the embryonic pancreas, and post-natally it is found in both beta - and δ (somatostatin) islet cells. This transcription factor complexes with multiple cofactors (Set9; NeuroD1; MafA; HMGA1) in its regulation of beta -cell division and insulin synthesis, and serves principally as an RNA pol II binding modulator. Over aa 91-283, human and mouse PDX-1 share 87% aa sequence identity.

Product Datasheets

Related Research Areas

• Diabetes
• Glucose Homeostasis
• Hepatic and Pancreatic Progenitor Cell Transcription Factors
• Homeodomain (Hox) Transcription Factors
• Mesenchymal Stem Cell Transcription Factors
• Pancreatic Endoderm
• Pancreatic Endoderm Cell Markers
• Transcription Factors and Regulators
• Transcription Factors in the Akt Pathway