Human/Mouse Phospho-STAT1 (Y701) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-STAT1 (Y701) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-a2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Phospho-STAT1 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN-a (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
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Detection of Human Phospho-STAT1 (Y701) by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT1
STAT1 (signal transducer and activator of transcription #1) is an 88 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT1 is phosphorylated at Y701 by receptor-associated Janus kinases (JAKs) leading to STAT1 dimerization and subsequent translocation to the nucleus to activate gene transcription.
Product Datasheets
Citations for Human/Mouse Phospho-STAT1 (Y701) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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C9ORF72 suppresses JAK-STAT mediated inflammation
Authors: Weilun Pang, Fenghua Hu
iScience
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Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC -
3-oxo-C12:2-HSL, quorum sensing molecule from human intestinal microbiota, inhibits pro-inflammatory pathways in immune cells via bitter taste receptors
Authors: G Coquant, D Aguanno, L Brot, C Belloir, J Delugeard, N Roger, HP Pham, L Briand, M Moreau, L de Sordi, V Carrière, JP Grill, S Thenet, P Seksik
Oncogene, 2022-06-08;12(1):9440.
Species: Mouse
Sample Types: Cell Lysates
Applications: Capillary Immunoblot -
Butyrate Prevents Induction of CXCL10 and Non-Canonical IRF9 Expression by Activated Human Intestinal Epithelial Cells via HDAC Inhibition
Authors: SGPJ Korsten, L Peracic, LMB van Groeni, MAP Diks, H Vromans, J Garssen, LEM Willemsen
International Journal of Molecular Sciences, 2022-04-02;23(7):.
Species: Human
Sample Types: Protein Extracts
Applications: Western Blot -
Kurarinone Attenuates Collagen-Induced Arthritis in Mice by Inhibiting Th1/Th17 Cell Responses and Oxidative Stress
Authors: KT Tang, CC Lin, SC Lin, JH Wang, SW Tsai
International Journal of Molecular Sciences, 2021-04-13;22(8):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
PD-L1 blockade enhances response of pancreatic ductal adenocarcinoma to radiotherapy
EMBO Mol Med, 2017-02-01;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes
Sci Rep, 2016-07-29;6(0):30448.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Molecular determinants of plaque size as an indicator of dengue virus attenuation
Sci Rep, 2016-05-17;6(0):26100.
Species: Hamster
Sample Types: Whole Cells
Applications: IHC -
Annexin V incorporated into influenza virus particles inhibits gamma interferon signaling and promotes viral replication.
Authors: Berri F, Haffar G, Le V, Sadewasser A, Paki K, Lina B, Wolff T, Riteau B
J Virol, 2014-07-16;88(19):11215-28.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells.
Authors: Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y
Mol. Ther., 2007-05-15;15(8):1531-6.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism
Authors: Lucia Napione, Marina Strasly, Claudia Meda, Stefania Mitola, Maria Alvaro, Gabriella Doronzo et al.
Angiogenesis
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First antibody: 1:2000, 4 oC, overnight
Second antibody: 1:5000, room temperature, 1 h
