Home / PHOX2B / Human/Mouse PHOX2B Antibody

Human/Mouse PHOX2B Antibody

Catalog #: AF4940
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4940
AF4940-SP
PHOX2B in IMR-32 Human Cell Line.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PHOX2B Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human PHOX2B in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PHOX2B
Met1-Glu94
Accession # Q99453
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry PHOX2B antibody in IMR-32 Human Cell Line by Immunocytochemistry (ICC). View Larger

PHOX2B in IMR-32 Human Cell Line. PHOX2B was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human PHOX2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4940) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PHOX2B

PHOX2B (Paired mesoderm homeobox protein 2B; also NBPhox) is a 33-35 kDa member of the paired homeobox family of transcription factors. It is not actually a mesoderm-associated protein, but is instead apparently restricted to neuronal precursors and mature neurons. It is found in glutamatergic neurons of the NTS and in neurons that demarcate the respiratory chemoreception pathway. In addition, PHOX2B is essential to the development of noradrenergic adrenal chromaffin and sympathetic motor neurons. Human PHOX2B is 314 amino acids (aa) in length. It contains one DNA binding homeobox domain (aa 98-157), and two poly-Ala sequences, one between aa 159-167, and another between aa 241-260. Alanine extensions involving anywhere from 5 to 11 Ala residues may exist. These change the nature of the PHOX2B molecule. While wild-type PHOX2B is nuclear, Ala extensions promote its retention in the cytoplasm with loss of activity. PHOX2B may potentially form oligomers with itself, or it paralogue, PHOX2A. Human and mouse PHOX2B are identical in aa sequence.

Long Name
Paired-like Homeobox 2b
Entrez Gene IDs
8929 (Human); 18935 (Mouse); 364152 (Rat)
Alternate Names
NBPhox; NBPhoxPhox2b; Neuroblastoma Phox; paired mesoderm homeobox 2b; paired mesoderm homeobox protein 2B; paired-like homeobox 2bNBLST2; PHOX2B homeodomain protein; PHOX2B; PMX2B; PMX2Bneuroblastoma paired-type homeobox protein

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Citations for Human/Mouse PHOX2B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Mapping of extrinsic innervation of the gastrointestinal tract in the mouse embryo
    Authors: X Niu, L Liu, T Wang, X Chuan, Q Yu, M Du, Y Gu, L Wang
    J. Neurosci., 2020-07-20;0(0):.
  2. Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells
    Authors: Tao, Y;Li, X;Dong, Q;Kong, L;Petersen, AJ;Yan, Y;Xu, K;Zima, S;Li, Y;Schmidt, DK;Ayala, M;Mathivanan, S;Sousa, AMM;Chang, Q;Zhang, SC;
    Nature biotechnology
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Flow Cytometry
  3. A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia
    Authors: Laddach, A;Chng, SH;Lasrado, R;Progatzky, F;Shapiro, M;Erickson, A;Sampedro Castaneda, M;Artemov, AV;Bon-Frauches, AC;Amaniti, EM;Kleinjung, J;Boeing, S;Ultanir, S;Adameyko, I;Pachnis, V;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Oral Sensory Neurons of the Geniculate Ganglion That Express Tyrosine Hydroxylase Comprise a Subpopulation That Contacts Type II and Type III Taste Bud Cells
    Authors: Tao Tang, Brian A. Pierchala
    eNeuro
  5. DHODH is an independent prognostic marker and potent therapeutic target in neuroblastoma
    Authors: Thale Kristin Olsen, Cecilia Dyberg, Bethel Tesfai Embaie, Adele Alchahin, Jelena Milosevic, Jane Ding et al.
    JCI Insight
  6. BET and CDK Inhibition Reveal Differences in the Proliferation Control of Sympathetic Ganglion Neuroblasts and Adrenal Chromaffin Cells
    Authors: J Sriha, C Louis-Bren, C Pierre-Eug, S Baulande, V Raynal, A Kramdi, I Adameyko, U Ernsberger, T Deller, O Delattre, I Janoueix-L, H Rohrer
    Cancers, 2022-06-01;14(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3
    Authors: CM Wright, S Schneider, KM Smith-Edwa, F Mafra, AJL Leembrugge, MV Gonzalez, DR Kothakapa, JB Anderson, BA Maguire, T Gao, TA Missall, MJ Howard, JC Bornstein, BM Davis, RO Heuckeroth
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-11;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Cell non-autonomous requirement of p75 in the development of geniculate oral sensory neurons
    Authors: T Tang, CR Donnelly, AA Shah, RM Bradley, CM Mistretta, BA Pierchala
    Scientific Reports, 2020-12-17;10(1):22117.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. The Human and Mouse Enteric Nervous System at Single-Cell Resolution
    Authors: Eugene Drokhlyansky, Christopher S. Smillie, Nicholas Van Wittenberghe, Maria Ericsson, Gabriel K. Griffin, Gokcen Eraslan et al.
    Cell
  10. Disordered breathing in a mouse model of Dravet syndrome
    Authors: Fu-Shan Kuo, Colin M Cleary, Joseph J LoTurco, Xinnian Chen, Daniel K Mulkey
    eLife

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