Human/Mouse PKC iota / lambda Antibody

Catalog #: MAB4465 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4465
MAB4465-SP

Detection of Human/Mouse PKC iota / lambda by Western Blot.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PKC iota / lambda Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse PKC iota / lambda in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 450401

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PKC iota
Ile455-Val596
Accession # P41743

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse PKC iota / lambda by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, DU145 human prostate carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PKC iota/lambda Monoclonal Antibody (Catalog # MAB4465) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKC iota/lambda at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC iota/lambda

Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their cofactor requirements: conventional PKC isoforms ( alpha, beta _Ι, beta _II, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms (δ, epsilon, eta, and theta ), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota / lambda, and zeta ), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC iota is highly expressed in brain and lung but is also expressed at lower levels in many tissues including pancreatic islets. It is the catalytic component of the Par3-Par6-atypical PKC complex, a key regulator of the formation and maintenance of epithelial cell polarity, adherens junctions, and tight junctions. PKC iota has also been shown to be critical for Ras-mediated transformation, invasion, and anchorage‑independent growth of intestinal epithelial cells. The rodent homolog is termed PKC lambda and is 95% homologous to PKC iota.

Long Name

Protein Kinase C iota

Alternate Names

aPKC-lambda/iota;Atypical protein kinase C-lambda/iota;nPKC-iota;PRKC-lambda/iota;Protein kinase C iota type;protein kinase C, iota;protein kinase C, lambda; PKC iota/lambda; PRKCI; PRKCL