Human/Mouse PKN1 Antibody

Catalog #: MAB6100 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6100
MAB6100-SP

Detection of Human and Mouse PKN1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PKN1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse PKN1 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 540421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PKN1
Ile215-Thr388
Accession # Q16512

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse PKN1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKN1 Monoclonal Antibody (Catalog # MAB6100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKN1

PKN1 (serine/threonine protein kinase N1), also known as PKN or PRK, is a ubiquitous 120 kDa cytoplasmic kinase of the PKC superfamily. The 942 amino acid (aa) PKN1 contains three HR1 repeats (aa 34‑263), a C2 domain (aa 325‑461), and a protein kinase domain (aa 615‑874). PKN1 can be activated by proteolysis, lipid interaction, or Rho G proteins, and is a downstream effector of RhoA. It promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. In vivo, PKN1 phosphorylates histone H3 at the Thr11 position.

Long Name

Serine/Threonine Protein Kinase N1

Entrez Gene IDs

5585 (Human); 320795 (Mouse); 29355 (Rat)

Alternate Names

DBK; EC 2.7.11; MGC46204; PAK1; PAK1Serine-threonine protein kinase N; PKN; PKN1; PKN-ALPHA; PKNPKN-ALPHA; PRK1; PRK1PAK-1; PRKCL1; PRKCL1protein kinase C-related kinase 1; Protease-activated kinase 1; Protein kinase C-like 1EC 2.7.11.13; Protein kinase C-like PKN; protein kinase N1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine/threonine protein kinase N; serine/threonine-protein kinase N1; serine-threonine kinase N