Human/Mouse PLC-beta 1 Antibody

Catalog #: AF4466 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4466
AF4466-SP

Detection of Human PLC‑ beta 1 by Western Blot.

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Human/Mouse PLC-beta 1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse PLC-beta 1, but does not detect recombinant human (rh) PLC-beta 2, rhPLC-beta 3, or rhPLC-beta 4 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PLC-beta 1
Lys27-Met245
Accession # Q9NQ66

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PLC‑ beta 1 by Western Blot. Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Human/Mouse PLC‑ beta 1 by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC-beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 1 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLC-beta 1

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 1 (Phospholipase C beta-1) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4, 5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. Alternative splicing results in two transcript variants encoding two isoforms, PLC-beta 1A and PLC-beta 1B. PLC-beta 1A is preferentially expressed in the cytosol, while PLC-beta 1B is predominantly expressed in the nucleus. The region used as immunogen is 100% identical in both isoforms.

Long Name

Phospholipase C beta 1

Entrez Gene IDs

23236 (Human); 18795 (Mouse); 24654 (Rat)

Alternate Names

EC 3.1.4.11; EIEE12; FLJ45792; inositoltrisphosphohydrolase; KIAA05811-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 1; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; Phosphoinositide phospholipase C-beta-1; phospholipase C, beta 1 (phosphoinositide-specific); Phospholipase C-beta-1; Phospholipase C-I; PI-PLC; PLC-154; PLC154,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1; PLCB1; PLCB1A; PLCB1B; PLCbeta 1; PLC-beta 1; PLC-beta-1; PLC-I; PLC-I1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; triphosphoinositide phosphodiesterase