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Human/Mouse Plexin C1 Antibody

Catalog #: MAB3887
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3887
MAB3887-SP
Plexin C1 in Human Melanoma.
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Plexin C1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Plexin C1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Plexin A4, B1, B2, B3, D1, recombinant mouse Plexin A1, A2, or A3 is observed.
Source
Monoclonal Mouse IgG2B Clone # 544232
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Plexin C1
Ile49-Ser161
Accession # O60486
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Plexin C1 antibody in Human Melanoma by Immunohistochemistry (IHC-P). View Larger

Plexin C1 in Human Melanoma. Plexin C1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Plexin C1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Plexin C1 in Mouse Embryo. Plexin C1 was detected in immersion fixed frozen sections of mouse embryonic gut (12 d.p.c.) using Mouse Anti-Human Plexin C1 Monoclonal Antibody (Catalog # MAB3887) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin C1

Plexin C1 (also VESPR and CD232) is a 210 kDa member of the plexin-C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells and neutrophils. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature human Plexin C1 is 1534 amino acids (aa) in length. It contains a 910 aa extracellular domain (ECD) and a 603 aa cytoplasmic region. The ECD contains one Sema-domain (aa 49-440), two PSI domains (aa 454-605) and two IPT regions (aa 664-842). Over aa 49-161, human Plexin C1 shares 92% aa identity with mouse Plexin C1.

Entrez Gene IDs
10154 (Human); 54712 (Mouse); 362873 (Rat)
Alternate Names
CD232 antigen; CD232; plexin (semaphorin receptor); Plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor

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Citations for Human/Mouse Plexin C1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Unraveling the transcriptional determinants of Liver Sinusoidal Endothelial Cell specialization
    Authors: de Haan W, Oie CI, Benkheil M et al.
    Am. J. Physiol. Gastrointest. Liver Physiol.
  2. In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia
    Authors: Viventi S, Frausin S, Howden SE et al.
    Stem cells translational medicine
  3. Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
    Authors: Myster F, Palmeira L, Sorel O, Bouillenne F, DePauw E, Schwartz-Cornil I, Vanderplasschen A, Dewals B
    J Virol, 2015-01-14;89(7):3630-47.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Intestinal epithelial cell-derived semaphorin 7A negatively regulates development of colitis via alphavbeta1 integrin.
    Authors: Kang S, Okuno T, Takegahara N, Takamatsu H, Nojima S, Kimura T, Yoshida Y, Ito D, Ohmae S, You DJ, Toyofuku T, Jang MH, Kumanogoh A
    J. Immunol., 2011-12-23;188(3):1108-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  5. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
    Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
    Sci Rep, 2018-01-12;8(1):603.

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Reviews for Human/Mouse Plexin C1 Antibody

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Human/Mouse Plexin C1 Antibody
By Anonymous on 11/12/2021
Application: IHC Sample Tested: Pancreatic cancer tissue Species: Mouse
Immunohistochemistry Human/Mouse Plexin C1 Antibody MAB3887