Human/Mouse/Primate BMP-9 Antibody

Name: Human/Mouse/Primate BMP-9 Antibody
Brand: R&D Systems
SKU: MAB3209
Rating: 5 (1 reviews)

Catalog #: MAB3209
14 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3209-100
MAB3209-500
MAB3209-SP

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody.

2 Images

All BMP-9 Antibodies

Product Details

Citations (14)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Primate BMP-9 Antibody Summary

Species Reactivity

Human, Mouse, Primate

Specificity

Detects human and primate BMP‑9 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP‑1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) BMP‑9 and 10% cross‑reactivity with rmBMP-10 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 360107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BMP-9
Ser320-Arg429
Accession # Q9UK05

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human/Primate BMP-9 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human/Primate BMP‑9 Biotinylated Antibody (Catalog # BAF3209)

Standard: Recombinant Human BMP-9 Protein (Catalog # 3209-BP)

Neutralization

Measured by its ability to neutralize BMP‑9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 0.01-0.04 µg/mL in the presence of 5 ng/mL Recombinant Human BMP‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse/Primate BMP-9 Monoclonal Antibody (Catalog # MAB3209). The ND₅₀ is typically 0.01-0.04 µg/mL.

Western Blot

View Larger

Detection of BMP-9 by Western Blot ENG is required for BMP9-mediated SMAD1/5/8 phosphorylation in endothelial cells.A) HUVECs were reversed-transfected in a 96-well plate with Mock, Random or ENG siRNAs for 48 hours then serum-starved for 3 hours prior to stimulation with BMP9 (0.1 ng/ml) (+) or PBS (−). Levels of ENG, pSMAD1/5/8, SMAD1 and beta -Actin in the total cell extracts were measured by western blotting. (B–D) ECs were dispensed in each well of a 96-well plate (HUVEC, HPAEC, HAEC –2500 cells/well, MS1–1250 cells/well) in serum free media. Cells were incubated with antibodies at various concentrations for 1 hour and stimulated with BMP9 (0.1 ng/ml) (+) or PBS (−) for 30 min prior to lysis. Levels of pSMAD1 normalized for levels of total SMAD1 were monitored using a SMAD1/pSMAD1 MSD assay. The following antibodies were used: rIgG2a (rat IgG2a isotype control), M999 (rat IgG2a anti-human ENG), hIgG2 (human IgG2 isotype control), cSN6j (human IgG2 anti-human ENG), Ab3209 (mouse IgG2b anti-human BMP9), M1041-43 (rat IgG2a anti-mouse ENG). * Antibody concentrations of Ab3209 used were 10 times lower than other antibodies. Results are the mean +/− standard deviation of technical triplicates (** = P Value <10E-04; *** = P value <10E-03). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0050920), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

References

Chen, D. et al. (2004) Growth Factors 22:233.
Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
Song, J.J. et al. (1995) Endocrinology 136:4293.
Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
Lopez-Coviella, I. et al. (2000) Science 289:313.
Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6984.
Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
Ploemacher, R.E. et al. (1999) Leukemia 13:428.

Long Name

Bone Morphogenetic Protein 9

Entrez Gene IDs

2658 (Human); 12165 (Mouse)

Alternate Names

BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Primate BMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
Filter your results:

Filter by:

BMP-9 mediates fibroproliferation in fibrodysplasia ossificans progressiva through TGF-? signaling
Authors: Zhao, C;Inada, Y;Motoike, S;Kamiya, D;Hino, K;Ikeya, M;
EMBO molecular medicine
Species: Transgenic Mouse
Sample Types: In Vivo
Applications: Neutralization
CDK6-mediated endothelial cell cycle acceleration drives arteriovenous malformations in hereditary hemorrhagic telangiectasia
Authors: Dinakaran, S;Zhao, H;Tang, Y;Wang, Z;Ruiz, S;Nomura-Kitabayashi, A;Blanc, L;Faughnan, ME;Marambaud, P;
bioRxiv : the preprint server for biology
Species: Mouse, Transgenic Mouse
Sample Types: In Vivo
Applications: Neutralization
Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis
Authors: NE Owen, GJ Alexander, S Sen, K Bunclark, G Polwarth, J Pepke-Zaba, AP Davenport, NW Morrell, PD Upton
EBioMedicine, 2020-05-23;56(0):102794.
Species: Human
Sample Types: Plasma
Applications: ELISA (detection)
Differential Consequences of Bmp9 Deletion on Sinusoidal Endothelial Cell Differentiation and Liver Fibrosis in 129/Ola and C57BL/6 Mice
Authors: A Desroches-, E Tillet, N Ricard, M Ouarné, C Mallet, JJ Feige, S Bailly
Cells, 2019-09-13;8(9):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Capture
Identification of rare sequence variation underlying heritable pulmonary arterial hypertension
Authors: S Gräf, M Haimel, M Bleda, C Hadinnapol, L Southgate, W Li, J Hodgson, B Liu, RM Salmon, M Southwood, RD Machado, JM Martin, CM Treacy, K Yates, LC Daugherty, O Shamardina, D Whitehorn, S Holden, M Aldred, HJ Bogaard, C Church, G Coghlan, R Condliffe, PA Corris, C Danesino, M Eyries, H Gall, S Ghio, HA Ghofrani, JSR Gibbs, B Girerd, AC Houweling, L Howard, M Humbert, DG Kiely, G Kovacs, RV MacKenzie, S Moledina, D Montani, M Newnham, A Olschewski, H Olschewski, AJ Peacock, J Pepke-Zaba, I Prokopenko, CJ Rhodes, L Scelsi, W Seeger, F Soubrier, DF Stein, J Suntharali, EM Swietlik, MR Toshner, DA van Heel, A Vonk Noord, Q Waisfisz, J Wharton, SJ Wort, WH Ouwehand, N Soranzo, A Lawrie, PD Upton, MR Wilkins, RC Trembath, NW Morrell
Nat Commun, 2018-04-12;9(1):1416.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
BMP-9 interferes with liver regeneration and promotes liver fibrosis
Authors: K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke
Gut, 2017-03-23;0(0):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development (Capture)
A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
Sci Rep, 2016-11-22;5(0):37366.
Species: Mouse
Sample Types: In Vivo, Serum
Applications: ELISA Development (Detection), Neutralization
Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis
Authors: BR Yoon, YH Chung, SJ Yoo, K Kawara, J Kim, IS Yoo, CG Park, SW Kang, WW Lee
J. Biol. Chem, 2015-12-23;291(8):4048-57.
Species: Human
Sample Types: Protein
Applications: ELISA Development (Capture)
Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains
Authors: Y Kienast, U Jucknischk, S Scheiblich, M Thier, M de Wouters, A Haas, C Lehmann, V Brand, D Bernicke, K Honold, S Lorenz
J. Biol. Chem, 2015-12-16;291(7):3395-410.
Species: Human
Sample Types: Protein
Applications: ELISA Development (Capture)
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
Nat Genet, 2012-12-02;45(1):34-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
Blood, 2012-05-07;119(25):6162-71.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
Authors: van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
J. Biol. Chem., 2012-04-05;287(22):18551-61.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
Blood, 2012-04-03;119(21):5037-47.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot

A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Authors: Herrera B, Inman GJ
BMC Cell Biol., 2009-03-19;10(0):20.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization