Human/Mouse/Primate BMP-9 Antibody

Name: Human/Mouse/Primate BMP-9 Antibody
Brand: R&D Systems
SKU: MAB3209
Rating: 5 (1 reviews)

Catalog #: MAB3209
13 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3209-100
MAB3209-500
MAB3209-SP

Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody.

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Citations (13)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Primate BMP-9 Antibody Summary

Species Reactivity

Human, Mouse, Primate

Specificity

Detects human and primate BMP‑9 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP‑1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) BMP‑9 and 10% cross‑reactivity with rmBMP-10 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 360107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BMP-9
Ser320-Arg429
Accession # Q9UK05

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human/Primate BMP-9 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human/Primate BMP‑9 Biotinylated Antibody (Catalog # BAF3209)

Standard: Recombinant Human BMP-9 Protein (Catalog # 3209-BP)

Neutralization

Measured by its ability to neutralize BMP‑9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 0.01-0.04 µg/mL in the presence of 5 ng/mL Recombinant Human BMP‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse/Primate BMP-9 Monoclonal Antibody (Catalog # MAB3209). The ND₅₀ is typically 0.01-0.04 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

References

Chen, D. et al. (2004) Growth Factors 22:233.
Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
Song, J.J. et al. (1995) Endocrinology 136:4293.
Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
Lopez-Coviella, I. et al. (2000) Science 289:313.
Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6984.
Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
Ploemacher, R.E. et al. (1999) Leukemia 13:428.

Long Name

Bone Morphogenetic Protein 9

Entrez Gene IDs

2658 (Human); 12165 (Mouse)

Alternate Names

BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Primate BMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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CDK6-mediated endothelial cell cycle acceleration drives arteriovenous malformations in hereditary hemorrhagic telangiectasia
Authors: Dinakaran, S;Zhao, H;Tang, Y;Wang, Z;Ruiz, S;Nomura-Kitabayashi, A;Blanc, L;Faughnan, ME;Marambaud, P;
bioRxiv : the preprint server for biology
Species: Mouse, Transgenic Mouse
Sample Types: In Vivo
Applications: Neutralization
Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis
Authors: NE Owen, GJ Alexander, S Sen, K Bunclark, G Polwarth, J Pepke-Zaba, AP Davenport, NW Morrell, PD Upton
EBioMedicine, 2020-05-23;56(0):102794.
Species: Human
Sample Types: Plasma
Applications: ELISA (detection)
Differential Consequences of Bmp9 Deletion on Sinusoidal Endothelial Cell Differentiation and Liver Fibrosis in 129/Ola and C57BL/6 Mice
Authors: A Desroches-, E Tillet, N Ricard, M Ouarné, C Mallet, JJ Feige, S Bailly
Cells, 2019-09-13;8(9):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Capture
Identification of rare sequence variation underlying heritable pulmonary arterial hypertension
Authors: S Gräf, M Haimel, M Bleda, C Hadinnapol, L Southgate, W Li, J Hodgson, B Liu, RM Salmon, M Southwood, RD Machado, JM Martin, CM Treacy, K Yates, LC Daugherty, O Shamardina, D Whitehorn, S Holden, M Aldred, HJ Bogaard, C Church, G Coghlan, R Condliffe, PA Corris, C Danesino, M Eyries, H Gall, S Ghio, HA Ghofrani, JSR Gibbs, B Girerd, AC Houweling, L Howard, M Humbert, DG Kiely, G Kovacs, RV MacKenzie, S Moledina, D Montani, M Newnham, A Olschewski, H Olschewski, AJ Peacock, J Pepke-Zaba, I Prokopenko, CJ Rhodes, L Scelsi, W Seeger, F Soubrier, DF Stein, J Suntharali, EM Swietlik, MR Toshner, DA van Heel, A Vonk Noord, Q Waisfisz, J Wharton, SJ Wort, WH Ouwehand, N Soranzo, A Lawrie, PD Upton, MR Wilkins, RC Trembath, NW Morrell
Nat Commun, 2018-04-12;9(1):1416.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
BMP-9 interferes with liver regeneration and promotes liver fibrosis
Authors: K Breitkopf-, C Meyer, C König, H Gaitantzi, A Addante, M Thomas, E Wiercinska, C Cai, Q Li, F Wan, C Hellerbran, NA Valous, M Hahnel, C Ehlting, JG Bode, S Müller-Boh, U Klingmülle, J Altenöder, I Ilkavets, MJ Goumans, LJ Hawinkels, SJ Lee, M Wieland, C Mogler, MP Ebert, B Herrera, H Augustin, A Sánchez, S Dooley, P Ten Dijke
Gut, 2017-03-23;0(0):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development (Capture)
A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
Sci Rep, 2016-11-22;5(0):37366.
Species: Mouse
Sample Types: In Vivo, Serum
Applications: ELISA Development (Detection), Neutralization
Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis
Authors: BR Yoon, YH Chung, SJ Yoo, K Kawara, J Kim, IS Yoo, CG Park, SW Kang, WW Lee
J. Biol. Chem, 2015-12-23;291(8):4048-57.
Species: Human
Sample Types: Protein
Applications: ELISA Development (Capture)
Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains
Authors: Y Kienast, U Jucknischk, S Scheiblich, M Thier, M de Wouters, A Haas, C Lehmann, V Brand, D Bernicke, K Honold, S Lorenz
J. Biol. Chem, 2015-12-16;291(7):3395-410.
Species: Human
Sample Types: Protein
Applications: ELISA Development (Capture)
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
Nat Genet, 2012-12-02;45(1):34-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
Blood, 2012-05-07;119(25):6162-71.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
Authors: van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
J. Biol. Chem., 2012-04-05;287(22):18551-61.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
Blood, 2012-04-03;119(21):5037-47.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot

A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Authors: Herrera B, Inman GJ
BMC Cell Biol., 2009-03-19;10(0):20.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization