Human/Mouse PUM1 Antibody

Catalog #: MAB7628 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7628
MAB7628-SP

Detection of Human and Mouse PUM1 by Western Blot.

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All PUM1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PUM1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human PUM1 in ELISAs. Detects human and mouse PUM1 in Western Blots

Source

Monoclonal Mouse IgG₁ Clone # 856332

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PUM1
Pro230-Ala372
Accession # Q14671

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse PUM1 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, BG01V human embryonic stem cells, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PUM1 Mono-clonal Antibody (Catalog # MAB7628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PUM1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PUM1

PUM1 (Pumilio homolog 1/Pumilio-1) is a 130-140 kDa member of the PUF (PUmilio and FBF) family of RNA-binding proteins. It is ubiquitously expressed and appears to be involved in cell cycle regulation. Among other things, PUM1 is known to bind to the 3' UTR of p27 mRNA following cell activation. This results in a conformational change in the p27 transcript, allowing for miRNA binding and translational repression, followed by a decline in p27 availability and entry into S-phase of the cell cycle. Human PUM1 is 1186 amino acids (aa) in length. It contains N-terminal Ala-, Gln- and Ser-rich regions (aa 393-815), one PUM-HD region (aa 828-1168) and eight consecutive pumilio repeats (aa 726-1142). There are six utilized Ser/Thr phosphorylation sites and at least three isoform variants. One contains a five aa insert after Gly418, a second possesses an alternative start site 36 aa upstream of the standard site, and a third shows a deletion of aa 597-623 coupled to a two aa insertion after Gln950. Over aa 230-372, human PUM1 is identical to mouse PUM1 in amino acid sequence. Overall, human and mouse PUM1 share 99% aa sequence identity.

Long Name

Pumilio 1

Entrez Gene IDs

9698 (Human); 80912 (Mouse); 362609 (Rat)

Alternate Names

HSPUM; KIAA0099PUML1; PUM1; PUMH; PUMH1; PUMH1pumilio homolog 1; pumilio (Drosophila) homolog 1; pumilio homolog 1 (Drosophila); Pumilio-1; PUML1