Human/Mouse PVRIG Antibody

Catalog #: MAB9365 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9365-100
MAB9365-SP

Detection of Human and Mouse PVRIG by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PVRIG Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse PVRIG in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 969102

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human PVRIG synthtic peptide
Accession # Q6DKI7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse PVRIG by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and TK-1 mouse T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PVRIG at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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PVRIG in MOLT‑4 Human Cell Line. PVRIG was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PVRIG

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

References

Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Alternate Names

C7orf15; C7orf15MGC138295; CD112R; MGC104322; MGC138297; MGC2463; poliovirus receptor related immunoglobulin domain containing; Poliovirus receptor-related immunoglobulin domain-containing protein; PVRIG; transmembrane protein PVRIG