Human/Mouse/Rat Androgen R/NR3C4 Antibody

Catalog #: AF5876 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5876
AF5876-SP

Detection of Human Androgen R/NR3C4 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Androgen R/NR3C4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Androgen R/NR3C4 in direct ELISAs. Detects human, mouse and rat Androgen R/NR3C4 in Western blotting.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Androgen R/NR3C4
Thr660-Gln919
Accession # P10275

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Androgen R/NR3C4 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Androgen R/NR3C4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5876) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Androgen R/NR3C4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Androgen R/NR3C4

The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR. AR (Androgen receptor) is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, mascrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain (aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1‑538 that generates a 45 kDa isoform. AR does homodimerize, apparently with multiple isotypes. Over aa 661‑920, human and mouse are identical in aa sequence.

Long Name

Androgen Receptor

Entrez Gene IDs

367 (Human)

Alternate Names

AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy