Human/Mouse/Rat Arginase 1/ARG1 Antibody

Name: Human/Mouse/Rat Arginase 1/ARG1 Antibody
Brand: R&D Systems
SKU: AF5868
Rating: 3.5 (2 reviews)

Catalog #: AF5868
16 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5868
AF5868-SP

Detection of Human and Rat Arginase 1/ARG1 by Western Blot.

3 Images

All Arginase 1/ARG1 Antibodies

Product Details

Citations (16)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Arginase 1/ARG1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Arginase 1/ARG1
Met1-Lys322
Accession # P05089

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Simple Western

2.5 µg/mL

See below

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human Arginase 1/ARG1 (Catalog # 5868-AR), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human and Rat Arginase 1/ARG1 by Western Blot. Western blot shows lysates of human liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western

View Larger

Detection of Human, Mouse, and Rat Arginase 1/ARG1 by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot

View Larger

Detection of Mouse Arginase 1/ARG1/liver Arginase by Western Blot Sepsis induces polarization of M2-like macrophages.(a–e) Lung tissue and peritoneal cells from C57BL/6 J (a,c,d) and BALB/c (b,e) mice undergoing CLP and antibiotic treatment were harvested at the indicated time points. (a) mRNA expression of Cebpb (C/EBP beta ), Arg1 (arginase-1), Mrc1 (MR) and Igf1 in the total lungs were determined by RT-qPCR at indicated times after CLP (n≥4 mice per group). (b) Lungs obtained from six mice, either naive or CLP (day 10 after CLP), were pooled from two independent samples and CD11b+ cells were isolated. mRNA expression of Arg1 (arginase-1), Mrc1 (MR), Rentla (encoding Fizz1) in isolated CD11b+ cells were determined by RT-qPCR. (c) IGF-1, CCL17 and CCL22 concentrations in the lungs were determined by ELISA at indicated times after CLP (n≥3 mice per group). (d) Representative FACS plots and frequency of peritoneal CD206+F4/80+ macrophages at indicated times after CLP (n≥3 mice per group). (e) Representative western blot of Arg1 (Arginase-1) expression in the peritoneal cells at day 10 after CLP (n=9 for naive group and n=5 for CLP group). ND, not detected. *P<0.05, **P<0.01 and ***P<0.001 (one-way ANOVA result with Dunnett posthoc tests in a,c, two-tailed unpaired Student's t-test in b,d). Data are from one (b) and representative of two (a,c–e) independent experiments (mean±s.e.m. in a–d). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28374774), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arginase 1/ARG1

Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Long Name

Liver-Type Arginase

Entrez Gene IDs

383 (Human); 11846 (Mouse); 29221 (Rat)

Alternate Names

AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; EC:3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Arginase 1/ARG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Filter by:

Attenuation of postoperative adhesions using a modeled manual therapy
Authors: Geoffrey M. Bove, Susan L. Chapelle, Katherine E. Hanlon, Michael P. Diamond, David J. Mokler
PLOS ONE
Leishmanicidal and immunomodulatory properties of Brazilian green propolis extract (EPP-AFï¿½) and a gel formulation in a pre-clinical model
Authors: J Rebouças-S, NA Amorim, FH Jesus-Sant, JA de Lima, JB Lima, AA Berretta, VM Borges
Frontiers in Pharmacology, 2023-02-09;14(0):1013376.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exercise
Authors: T Komori, Y Morikawa
The Journal of Biological Chemistry, 2022-11-09;0(0):102686.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Cell-intrinsic Wnt4 ligand regulates mitochondrial oxidative phosphorylation in macrophages
Authors: M Tlili, H Acevedo, A Descoteaux, M Germain, KM Heinonen
The Journal of Biological Chemistry, 2022-06-25;0(0):102193.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
PD-L1 engagement on T cells promotes self-tolerance and suppression of neighboring macrophages and effector T cells in cancer
Authors: B Diskin, S Adam, MF Cassini, G Sanchez, M Liria, B Aykut, C Buttar, E Li, B Sundberg, RD Salas, R Chen, J Wang, M Kim, MS Farooq, S Nguy, C Fedele, KH Tang, T Chen, W Wang, M Hundeyin, JAK Rossi, E Kurz, MIU Haq, J Karlen, E Kruger, Z Sekendiz, D Wu, SAA Shadaloey, G Baptiste, G Werba, S Selvaraj, C Loomis, KK Wong, J Leinwand, G Miller
Nat. Immunol., 2020-03-09;21(4):442-454.
Species: Human, Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery
Authors: W Ren, J Hou, C Yang, H Wang, S Wu, Y Wu, X Zhao, C Lu
J. Exp. Clin. Cancer Res., 2019-02-08;38(1):62.
Species: Xenograft
Sample Types: Cell Lysates
Applications: Western Blot
The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease
Authors: H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering
Acta Neuropathol Commun, 2019-01-31;7(1):13.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Magnetic Field Changes Macrophage Phenotype
Authors: J Wosik, W Chen, K Qin, RM Ghobrial, JZ Kubiak, M Kloc
Biophys. J., 2018-04-24;114(8):2001-2013.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity
Authors: J Suwanpradi, M Shih, L Pontius, B Yang, A Birukova, E Guttman-Ya, DL Corcoran, LG Que, RM Tighe, AS MacLeod
J. Immunol., 2017-07-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Assessment of Preclinical Liver and Skeletal Muscle Biomarkers Following Clofibrate Administration in Wistar Rats
Authors: P Maliver, M Festag, M Bennecke, F Christen, B Bánfai, B Lenz, M Winter
Toxicol Pathol, 2017-05-09;0(0):1926233177072.
Species: Rat
Sample Types: Serum
Applications: ELISA Development (Capture)

IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population
Authors: DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh
Nat Commun, 2017-04-04;8(0):14919.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Candida albicans Chitin Increases Arginase-1 Activity in Human Macrophages, with an Impact on Macrophage Antimicrobial Functions
Authors: J Wagener, DM MacCallum, GD Brown, NA Gow
MBio, 2017-01-24;8(1):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

DAMP signaling is a key pathway inducing immune modulation after brain injury.
Authors: Liesz A, Dalpke A, Mracsko E, Antoine D, Roth S, Zhou W, Yang H, Na S, Akhisaroglu M, Fleming T, Eigenbrod T, Nawroth P, Tracey K, Veltkamp R
J Neurosci, 2015-01-14;35(2):583-98.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-kappa B inhibition
Authors: Dang-ke Li, Guang-hui Wang
Pharmaceutical Biology

Carbon ion radiotherapy boosts anti-tumour immune responses by inhibiting myeloid-derived suppressor cells in melanoma-bearing mice
Authors: Heng Zhou, Pengfei Yang, Haining Li, Liying Zhang, Jin Li, Tianyi Zhang et al.
Cell Death Discovery

Hsp70-containing extracellular vesicles are capable of activating of adaptive immunity in models of mouse melanoma and colon carcinoma
Authors: Elena Y. Komarova, Roman V. Suezov, Alina D. Nikotina, Nikolay D. Aksenov, Luiza A. Garaeva, Tatiana A. Shtam et al.
Scientific Reports