Human/Mouse/Rat ARHGAP1 Antibody

Catalog #: AF6407 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6407
AF6407-SP

Detection of Human, Mouse, and Rat ARHGAP1 by Western Blot.

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Human/Mouse/Rat ARHGAP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ARHGAP1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ARHGAP1
aa 1-62
Accession # Q07960

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat ARHGAP1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat ARHGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ARHGAP1 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARHGAP1

ARHGAP1 (also p50-RhoGAP and CDC42 GTPase-activating protein) is a 50 kDa member of the ARHGAP family of proteins. It is preferentially expressed in epithelium, and serves as a cytoplasmic protein that deactivates Rho-family GTPases. It does so by contributing an Arg residue (Arg282) to Cdc42 and RhoA, which activates their intrinsic GTPase activity and converts GTP-associated molecules into inactivating GDP molecules. Human ARHGAP1 is 439 amino acids (aa) in length and contains one CRAL-TRIO domain that binds small lipophilic molecules (aa 53‑218), an SH3 binding site (aa 228‑238) and one C‑terminal RhoGAP domain (aa 244‑431). There are phosphorylation sites at Ser51 and Tyr65. There is one potential splice form with a 24 aa substitution for aa 1‑150 coupled to a 26 aa substitution for aa 300‑439. Over aa 1‑62, human ARHGAP1 shares 96% aa identity with mouse ARHGAP1.

Long Name

Rho GTPase Activating Protein 1

Entrez Gene IDs

392 (Human); 228359 (Mouse); 311193 (Rat)

Alternate Names

ARHGAP1; CDC42 GTPase-activating protein; CDC42GAP; CDC42GAPp50-RhoGAP; GTPase-activating protein rhoOGAP; p50rhoGAP; Rho GTPase activating protein 1; rho GTPase-activating protein 1; RhoGAP; RHOGAP1Cdc42GAP; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1