Human/Mouse/Rat Bcl-10 Antibody

Catalog #: MAB2889 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2889
MAB2889-SP

Detection of Human/Mouse/Rat Bcl‑10 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Bcl-10 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat Bcl-10 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 384202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Bcl-10
Met1-Leu192
Accession # O95999

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Bcl‑10 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Bcl-10 Monoclonal Antibody (Catalog # MAB2889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl-10 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-10

B cell CLL/leukemia 10 (Bcl-10), a 233 amino acid, 26.2 kDa protein, is the cellular homolog of the equine herpesvirus-2 gene (E10). Bcl-10 is also known as CARD like apoptotic protein (CLAP), CED-3/ICH-1 prodomain homologous E10-like regulator (CIPER), celluar-E10 (c-E10), and CARD-containing molecule enhancing NF kappa B (CARMEN). Bcl-10 contains a caspase recruitment domain (CARD) and has been shown to induce apoptosis and to activate NF kappa B. Bcl-10 can interact with other CARD domain containing proteins including CARD9, 10, 11, and 14, which function as upstream regulators of NF kappa B signaling. Defects in Bcl‑10 may be involved in the pathogenesis of human malignancy. Over aa 1-192, human Bcl-10 is 92% and 93% aa identical to mouse and rat Bcl-10, respectively.

Long Name

B Cell Lymphoma/Leukemia 10

Entrez Gene IDs

8915 (Human)

Alternate Names

B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10; Bcl10; Bcl-10; CARD-containing apoptotic signaling protein; CARD-containing molecule enhancing NF-kappa-B; CARD-containing proapoptotic protein; CARD-like apoptotic protein; CARMEN; caspase-recruiting domain-containing protein; cCARMEN; c-E10; c-E10bcl-10; CED-3/ICH-1 prodomain homologous E10-like regulator; Cellular homolog of vCARMEN; cellular-E10; CIPER; CLAP; hCLAP; Mammalian CARD-containing adapter molecule E10; mE10; mE10CARD containing molecule enhancing NF-kB