Human/Mouse/Rat Blk Antibody

Catalog #: AF2679 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2679
AF2679-SP

Detection of Human/Mouse/Rat Blk by Western Blot.

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Human/Mouse/Rat Blk Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Blk in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Blk
Met1-Val63
Accession # P51451

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Blk by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Blk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2679) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Blk at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Blk

B-cell lymphocyte kinase (Blk) is a member of the Src family of non‑receptor tyrosine kinases. Constitutively active Blk induces malignant transformation of B-cell progenitors, suggesting a role for Blk in B-lineage cell differentiation and proliferation.

Long Name

B Lymphoid Tyrosine Kinase

Entrez Gene IDs

640 (Human); 12124 (Mouse); 364403 (Rat)

Alternate Names

B lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; Blk; EC 2.7.10.2; MGC10442; MODY11; p55-Blk; tyrosine-protein kinase Blk