Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antibody

Catalog #: AF2504
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2504
AF2504-SP

Detection of Human, Mouse, and Rat Carbonic Anhydrase XIV/CA14 by Western Blot.

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All Carbonic Anhydrase XIV/CA14 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects mouse Carbonic Anhydrase XIV/CA14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with rhCA1, -2, -3, -6, -8, -10, -13, recombinant mouse CA4, -9, and -12 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XIV/CA14
Ala16-Met290
Accession # Q9WVT6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Carbonic Anhydrase XIV (Catalog # 2504-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Carbonic Anhydrase XIV/CA14 by Western Blot. Western blot shows lysates of human, mouse, and rat brain (cerebellum) tissue and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse and Rat Carbonic Anhydrase XIV/CA14 by Simple Western^TM. Simple Western lane view shows lysates of mouse and rat brain (cerebellum) tissue and Hepa 1-6 mouse hepatoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/ CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XIV/CA14

Carbonic anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Mouse CA14 is a type I membrane enzyme highly expressed in the kidney and heart, followed by the skeletal muscle, brain, lung and liver (2). The deduced amino acid sequence of mouse CA14 consists of a signal peptide (residues 1 to 15), an ectodomain (residues 16 to 290), a transmembrane domain (residues 291 to 311) and a cytoplamic region (residues 32 to 337). rmCA14 corresponds to the ectodomain and has activity as described in Activity Assay Protocol.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Mori, K. et al. (1999) J. Biol. Chem. 274:15701.

Entrez Gene IDs

23632 (Human); 23831 (Mouse)

Alternate Names

CA14; Carbonate dehydratase XIV; Carbonic Anhydrase XIV; carbonic anhydrase XIVcarbonic anhydrase 14; carbonic dehydratase; CAXiV; CA-XIV; EC 4.2.1.1

Product Datasheets

Product Datasheet

COA

SDS

Citation for Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CO2 chemosensing in rat oesophagus.
Authors: Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
Gut, 2008-08-05;57(12):1654-64.
Species: Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr

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