Human/Mouse/Rat Casein Kinase 1 delta Antibody Summary
Arg316-Arg415
Accession # P48730
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Casein Kinase 1δ by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1d at approximately 49 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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Casein Kinase 1δ in C2C12 Mouse Cell Line. Casein Kinase 1d was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 1 delta
Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3 ,δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1δ phosphorylates p53 as well as MDM2 and seems to play a principal role in coordinating the effects of these two key regulator proteins on cell growth and genome integrity.
Product Datasheets
Citation for Human/Mouse/Rat Casein Kinase 1 delta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Dense core vesicle markers in CSF and cortical tissues of patients with Alzheimer’s disease
Authors: Neus Barranco, Virginia Plá, Daniel Alcolea, Irene Sánchez-Domínguez, Reiner Fischer-Colbrie, Isidro Ferrer et al.
Translational Neurodegeneration
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