Human/Mouse/Rat Casein Kinase 1 gamma Antibody

Catalog #: AF3186 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3186
AF3186-SP

Detection of Human/Mouse/Rat Casein Kinase 1 gamma by Western Blot.

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Human/Mouse/Rat Casein Kinase 1 gamma Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat CK1 gamma in Western blots. In Western blots, the immunogen is derived from a region of human CK1 gamma that is 97% to 98% identical to human CK1 gamma ² and CK1 gamma ³, so all three isoforms should be detected.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CK1 gamma
Val182-Tyr304
Accession # Q9HCP0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Casein Kinase 1 gamma by Western Blot. Western blot shows lysates of human, mouse and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CK1 gamma isoforms at approximately 45 to 51 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Immunohistochemistry

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Casein Kinase 1 gamma in Human Brain. Casein Kinase 1 gamma was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Sheep Anti-Human/Mouse/Rat Casein Kinase 1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3186) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Casein Kinase 1 gamma

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, beta, gamma ¹, gamma ², gamma ³, δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. The C-termini of the gamma isoforms carry putative palmitoylation sites unique within the CK1 family that are believed to anchor the proteins in the plasma membrane.

Entrez Gene IDs

53944 (Human); 214897 (Mouse); 64086 (Rat)

Alternate Names

Casein Kinase 1 gamma; casein kinase 1, gamma 1; casein kinase I isoform gamma-1; CKI-gamma 1; CSNK1G1; EC 2.7.11; EC 2.7.11.1