Home / Cathepsin X/Z/P / Human/Mouse/Rat Cathepsin X/Z/P Antibody

Human/Mouse/Rat Cathepsin X/Z/P Antibody

Catalog #: AF934
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF934
AF934-SP
Detection of Human, Mouse, and Rat Cathepsin X/Z/P by Western Blot.
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Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Cathepsin X/Z/P Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Cathepsin X/Z/P in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin X/Z/P
Ala21-Val303 (Gly23Val, Ser48Thr)
Accession # Q9UBR2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin X/Z/P (Catalog # 934-CY), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Cathepsin X/Z/P antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Cathepsin X/Z/P by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Cathepsin X/Z/P at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cathepsin X/Z/P antibody in Human Breast and Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Cathepsin X/Z/P in Human Breast and Breast Cancer Tissue. Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of normal human breast and breast cancer tissue using Goat Anti-Human/Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Cathepsin X/Z/P antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Cathepsin X/Z/P by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin X/Z/P at approximately 36 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References
  1. Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
  2. Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
  3. Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
  4. Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
  5. Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
  6. Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, R. et al. (2001) Biol. Chem. 382:839.
  8. Therrien, C. et al. (2001) Biochemistry 40:2702.
  9. Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, G. et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.
Entrez Gene IDs
1522 (Human); 64138 (Mouse)
Alternate Names
Cathepsin P; Cathepsin X/Z/P; Cathepsin Z; CTSX; CTSZ

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Citations for Human/Mouse/Rat Cathepsin X/Z/P Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation
    Authors: Calcagni' A, Staiano L, Zampelli N et al.
    Nature communications
  2. USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction
    Authors: A Hertel, LM Alves, H Dutz, G Tascher, F Bonn, M Kaulich, I Dikic, S Eimer, F Steinberg, A Bremm
    Cell Reports, 2022-12-06;41(10):111653.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ICC, Western Blot
  3. Cysteine Peptidase Cathepsin X as a Therapeutic Target for Simultaneous TLR3/4-mediated Microglia Activation
    Authors: Anja Pišlar, Biljana Božić Nedeljković, Mina Perić, Tanja Jakoš, Nace Zidar, Janko Kos
    Molecular Neurobiology
  4. Cathepsin X Activity Does Not Affect NK-Target Cell Synapse but Is Rather Distributed to Cytotoxic Granules
    Authors: Tanja Jakoš, Mateja Prunk, Anja Pišlar, Janko Kos
    International Journal of Molecular Sciences
  5. NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C
    Authors: Oliver B. Davis, Hijai R. Shin, Chun-Yan Lim, Emma Y. Wu, Matthew Kukurugya, Claire F. Maher et al.
    Developmental Cell
  6. Neuroinflammation-Induced Upregulation of Glial Cathepsin X Expression and Activity in vivo
    Authors: Anja Pišlar, Larisa Tratnjek, Gordana Glavan, Nace Zidar, Marko Živin, Janko Kos
    Frontiers in Molecular Neuroscience
  7. Upregulation of Cysteine Protease Cathepsin X in the 6-Hydroxydopamine Model of Parkinson’s Disease
    Authors: Anja Pišlar, Larisa Tratnjek, Gordana Glavan, Marko Živin, Janko Kos
    Frontiers in Molecular Neuroscience
  8. Localization patterns of cathepsins K and X and their predictive value in glioblastoma
    Authors: Barbara Breznik, Clara Limback, Andrej Porcnik, Andrej Blejec, Miha Koprivnikar Krajnc, Roman Bosnjak et al.
    Radiology and Oncology
  9. Cysteine cathepsins B, X and K expression in peri-arteriolar glioblastoma stem cell niches
    Authors: Barbara Breznik, Clara Limbaeck Stokin, Janko Kos, Mohammed Khurshed, Vashendriya V. V. Hira, Roman Bošnjak et al.
    Journal of Molecular Histology
  10. Increased expression and altered localization of cathepsin Z are associated with progression to jaundice stage in primary biliary cholangitis
    Authors: Y Aiba, K Harada, M Ito, T Suematsu, S Aishima, Y Hitomi, N Nishida, M Kawashima, M Takatsuki, S Eguchi, S Shimoda, H Nakamura, A Komori, S Abiru, S Nagaoka, K Migita, H Yatsuhashi, K Tokunaga, M Nakamura
    Sci Rep, 2018-08-07;8(1):11808.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  11. Cysteine cathepsins: Their biological and molecular significance in cancer stem cells
    Authors: P Anja, J Anahid, K Janko
    Semin. Cancer Biol., 2018-07-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Cathepsin X Cleaves Profilin 1 C-Terminal Tyr139 and Influences Clathrin-Mediated Endocytosis.
    Authors: Pecar Fonovic U, Kos J
    PLoS ONE, 2015-09-01;10(9):e0137217.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix.
    Authors: Akkari L, Gocheva V, Kester J, Hunter K, Quick M, Sevenich L, Wang H, Peters C, Tang L, Klimstra D, Reinheckel T, Joyce J
    Genes Dev, 2014-10-01;28(19):2134-50.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  14. Prognostic and predictive value of cathepsin X in serum from colorectal cancer patients.
    Authors: Vizin T, Christensen I, Wilhelmsen M, Nielsen H, Kos J
    BMC Cancer, 2014-04-13;14(0):259.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  15. Three-dimensional invasion of macrophages is mediated by cysteine cathepsins in protrusive podosomes.
    Authors: Jevnikar Z, Mirkovic B, Fonovic U, Zidar N, Svajger U, Kos J
    Eur J Immunol, 2012-11-07;42(12):3429-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC

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