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Human/Mouse/Rat CCR5 Fluorescein-conjugated Antibody

Catalog #: FAB1802F
4 Citations Datasheet / COA / SDS

Discontinued Product

FAB1802F has been discontinued.
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Detection of CCR5 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CCR5 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human CCR5 in Western blots and human,mouse, and rat CCR5 in flow cytometry. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
Source
Monoclonal Mouse IgG1 Clone # CTC5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with human CCR5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1802F) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Polyclonal Antibody (Catalog # AB-404-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse/Rat CCR5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1802F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References
  1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
Entrez Gene IDs
1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
Alternate Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

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Citations for Human/Mouse/Rat CCR5 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation
    Authors: JM Laufer, MA Hauser, I Kindinger, V Purvanov, A Pauli, DF Legler
    Cell Rep, 2019-10-22;29(4):995-1009.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Attenuated Macrophage Infiltration in Glomeruli of Aged Mice Resulting in Ameliorated Kidney Injury in Nephrotoxic Serum Nephritis
    Authors: Y Kaneko, T Cho, Y Sato, K Goto, S Yamamoto, S Goto, MP Madaio, I Narita
    J. Gerontol. A Biol. Sci. Med. Sci., 2018-08-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Interferon gamma-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with
    Authors: Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y
    Oncoimmunology, 2013-07-23;2(8):e25752.
    Species: Mouse
    Sample Types: Whole Cells
  4. Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection.
    Authors: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W
    J. Immunol., 2011-06-10;187(2):748-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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