Human/Mouse/Rat CDC25C Antibody

Detection of Human and Mouse CDC25C by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat CDC25C Monoclonal Antibody (Catalog # MAB4459) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CDC25C at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of CDC25C by Western Blot Knockdown ANXA1 Induces G2/M Cell Cycle Arrest in Glioma Cells via the PI3K/Akt Signaling Pathway. A: Inhibition of ANXA1 decreases the expression of cdc25C, cyclin B1 and cdc2, whereas cyclin D1 was not altered evidently. Western blot assay analysis was performed using anti-cdc25C, anti-cyclin B1, anti-cyclin D1, anti-beta -actin and anti-cdc2 antibodies. B: The relative amounts of cdc-25C, cyclin B1, cdc2 and Cyclin D1 were quantified by a densitometric analysis (ImageJ). C: Western blot analysis of PI3K subunit p110 alpha and p85, phosphorylation and total protein levels of Akt, GSK3 beta and beta -catenin after si-ANXA1 or si-NC transfection for 48 h. D: Quantitative charts of phosphorylation and the total level of Akt, PI3K subunit p110 alpha and p85, GSK3 beta and beta -catenin. E: Inhibition of ANXA1 has no effect on the expression of p-p65NF-kappa B and up-regulates the expression of cPLA2. Western blot assay analysis was performed using anti-p-p65NF-kappa B, anti-p65NF-kappa B, anti-cPLA2 and anti-beta -actin antibodies. F: The relative amounts of p-p65NF-kappa B and cPLA2 were quantified by a densitometric analysis (ImageJ). G: ELISA analysis of nuclear NF-kappa B activity. N = 9 per group. H: cPLA2 activity in control, si-NC and si-ANXA1 U87 cells. cPLA2 activity was determined as described in Methods. These results were expressed as the mean ± SD from three independent experiments, *p < 0.05, **p < 0.01 compared with si-NC group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33706561), licensed under a CC-BY license. Not internally tested by R&D Systems.