Home / Contactin-2/TAG1 / Human/Mouse/Rat Contactin-2/TAG1 Antibody

Human/Mouse/Rat Contactin-2/TAG1 Antibody

Catalog #: AF4439
43 Citations 2 Reviews Datasheet / COA / SDS
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AF4439
AF4439-SP
Detection of Human, Mouse, and Rat Contactin‑2/TAG1 by Western Blot.
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Citations (43)
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Reviews (2)
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Human/Mouse/Rat Contactin-2/TAG1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse and rat Contactin-2 in direct ELISAs. Detects human, mouse, and rat Contactin-2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Contactin-2/TAG1
Gln31-Ser1014
Accession # Q61330
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Contactin-2/TAG1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Contactin‑2/TAG1 by Western Blot. Western blot shows lysates of human cerebellum tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin-2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Contactin-2/TAG1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Contactin‑2/TAG1 in Mouse Embryo. Contactin-2/TAG1 was detected in immersion fixed frozen sections of mouse embryo (E13) using Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells in proximity to ribs. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Contactin‑2/TAG1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Contactin‑2/TAG1 by Simple WesternTM. Simple Western lane view shows lysates of mouse spinal cord tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin-2/TAG1 at approximately 162 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-2/TAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4439) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Mouse Human/Mouse/Rat Contactin-2/TAG1 Antibody by Western Blot View Larger

Detection of Mouse Human/Mouse/Rat Contactin-2/TAG1 Antibody by Western Blot Quantitative proteomics in Rab35 cKO P0 hippocampus.a Volcano plot of the TMT-based quantitative proteomes identifying the dysregulated proteins in Rab35 cKO hippocampus in comparison with the control hippocampus (n = 5 mice per genotype). b Number of proteins identified as significantly dysregulated and as either membrane traffic-related or neuronal migration-related. c, d Western blot analysis of control and Rab35 cKO P0 hippocampi using anti-contactin-2, anti-CHL1, and anti-actin antibodies. e, f Quantification of contactin-2 (c) and CHL1 (d) protein levels in control and Rab35 cKO P0 hippocampi. Band intensities of the indicated proteins were normalized to those of actin (n = 9 mice per genotype). Unpaired Student’s t-test; e, p = 0.0153; f, p = 0.0095. g, h Levels of contactin-2 (g) and CHL1 (h) were quantified by targeted MS using the PRM method (n = 5 mice per genotype). Unpaired Student’s t-test; gp = 0.0053; hp = 0.0229. i Western blot analysis of control and Rab35 cKO P0 hippocampus using anti-N-cadherin and anti-actin antibodies. j Quantification of N-cadherin protein levels in the control and Rab35 cKO P0 hippocampus (n = 9 mice per genotypes). Unpaired Student’s t-test, p = 0.9020. k Representative images of DIV 2 hippocampal primary neurons stained for contactin-2 (green), rhodamine-phalloidin (magenta) and DAPI (blue). Scale bar, 20 μm. l Quantification of contactin-2 intensity at the somatic plasma membrane in control (n = 4) and Rab35-deficient (n = 4) cells. Thirty neurons from four different cultures per genotype were analyzed. Mann–Whitney U-test, p = 0.0286. Data represent the mean ± SEM; n.s. not significant (p > 0.05); *p < 0.05; **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37085665), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1-3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97%, and 77% aa sequence identity with human, rat, and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3-5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3-6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits contactin-associated protein 2 (caspr2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7-10). The axonal Contactin-2 interacts in cis with caspr2 and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

References
  1. Wolfer, D. and R.J. Giger (1994) Swissprot Accession # Q61330.
  2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
  3. Karagogeos, D. (2003) Front. Biosci. 8:s1304.
  4. Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
  5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
  6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
  7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
  8. Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
  9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
Entrez Gene IDs
6900 (Human); 21367 (Mouse)
Alternate Names
Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein

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Citations for Human/Mouse/Rat Contactin-2/TAG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Long-COVID cognitive impairments and reproductive hormone deficits in men may stem from GnRH neuronal death
    Authors: Sauve F, Nampoothiri S, Clarke SA et al.
    EBioMedicine
  2. Serum exosomal coronin 1A and dynamin 2 as neural tube defect biomarkers
    Authors: Yanfu Wang, Ling Ma, Shanshan Jia, Dan Liu, Hui Gu, Xiaowei Wei et al.
    Journal of Molecular Medicine
  3. Neural Stem Cells Direct Axon Guidance via Their Radial Fiber Scaffold
    Authors: Navjot Kaur, Wenqi Han, Zhuo Li, M. Pilar Madrigal, Sungbo Shim, Sirisha Pochareddy et al.
    Neuron
  4. Variation in a Left Ventricle–Specific Hand1 Enhancer Impairs GATA Transcription Factor Binding and Disrupts Conduction System Development and Function
    Authors: Joshua W. Vincentz, Beth A. Firulli, Kevin P. Toolan, Dan E. Arking, Nona Sotoodehnia, Juyi Wan et al.
    Circulation Research
  5. Myocardial Notch Signaling Reprograms Cardiomyocytes to a Conduction-Like Phenotype
    Authors: Stacey Rentschler, Alberta H. Yen, Jia Lu, Nataliya B. Petrenko, Min Min Lu, Lauren J. Manderfield et al.
    Circulation
  6. Transient Notch Activation Induces Long-Term Gene Expression Changes Leading to Sick Sinus Syndrome in Mice
    Authors: Yun Qiao, Catherine Lipovsky, Stephanie Hicks, Somya Bhatnagar, Gang Li, Aditi Khandekar et al.
    Circulation Research
  7. Partial and complete loss of myosin binding protein H-like cause cardiac conduction defects
    Authors: David Y. Barefield, Sean Yamakawa, Ibrahim Tahtah, Jordan J. Sell, Michael Broman, Brigitte Laforest et al.
    Journal of Molecular and Cellular Cardiology
  8. Overlapping transcriptional programs promote survival and axonal regeneration of injured retinal ganglion cells
    Authors: Anne Jacobi, Nicholas M. Tran, Wenjun Yan, Inbal Benhar, Feng Tian, Rebecca Schaffer et al.
    Neuron
  9. DDX3X Suppresses the Susceptibility of Hindbrain Lineages to Medulloblastoma
    Authors: Deanna M. Patmore, Amir Jassim, Erica Nathan, Reuben J. Gilbertson, Daniel Tahan, Nadin Hoffmann et al.
    Developmental Cell
  10. Defects in the Alternative Splicing-Dependent Regulation of REST Cause Deafness
    Authors: Nakano Y, Kelly MC, Rehman AU et al.
    Cell
  11. ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes
    Authors: Akshay Shekhar, Xianming Lin, Bin Lin, Fang-Yu Liu, Jie Zhang, Alireza Khodadadi-Jamayran et al.
    Scientific Reports
  12. Plexin-B2 controls the timing of differentiation and the motility of cerebellar granule neurons
    Authors: Van Battum E, Heitz-Marchaland C, Zagar Y Et al.
    eLife
  13. Ascending midbrain dopaminergic axons require descending GAD65 axon fascicles for normal pathfinding
    Authors: Claudia M. García-Peña, Minkyung Kim, Daniela Frade-Pérez, Daniela Ávila-González, Elisa Téllez, Grant S. Mastick et al.
    Frontiers in Neuroanatomy
  14. Neural cell adhesion molecule is required for ventricular conduction system development
    Authors: Camila Delgado, Lei Bu, Jie Zhang, Fang-Yu Liu, Joseph Sall, Feng-Xia Liang et al.
    Development
  15. GDE2-Dependent Activation of Canonical Wnt Signaling in Neurons Regulates Oligodendrocyte Maturation
    Authors: Bo-Ran Choi, Clinton Cave, Chan Hyun Na, Shanthini Sockanathan
    Cell Reports
  16. A tug of war between DCC and ROBO1 signaling during commissural axon guidance
    Authors: Brianna Dailey-Krempel, Andrew L. Martin, Ha-Neul Jo, Harald J. Junge, Zhe Chen
    Cell Reports
  17. beta -Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses
    Authors: Stephanie Hartmann, Fang Zheng, Michele C. Kyncl, Sandra Karch, Kerstin Voelkl, Benedikt Zott et al.
    The Journal of Neuroscience
  18. RAB35 is required for murine hippocampal development and functions by regulating neuronal cell distribution
    Authors: Ikuko Maejima, Taichi Hara, Satoshi Tsukamoto, Hiroyuki Koizumi, Takeshi Kawauchi, Tomoko Akuzawa et al.
    Communications Biology
  19. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM
    Authors: Yilin Tai, Nicholas B. Gallo, Minghui Wang, Jia-Ray Yu, Linda Van Aelst
    Neuron
  20. Transcriptional regulation of the postnatal cardiac conduction system heterogeneity
    Authors: Oh, Y;Abid, R;Dababneh, S;Bakr, M;Aslani, T;Cook, DP;Vanderhyden, BC;Park, JG;Munshi, NV;Hui, CC;Kim, KH;
    Nature communications
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  21. Long-COVID cognitive impairments and reproductive hormone deficits in men may stem from GnRH neuronal death
    Authors: Sauve F, Nampoothiri S, Clarke SA et al.
    EBioMedicine
  22. Histological Analysis of a Mouse Model of the 22q11.2 Microdeletion Syndrome
    Authors: Tabata, H;Mori, D;Matsuki, T;Yoshizaki, K;Asai, M;Nakayama, A;Ozaki, N;Nagata, KI;
    Biomolecules
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  23. Defective Jagged1 signaling impacts GnRH development and contributes to congenital hypogonadotropic hypogonadism
    Authors: L Cotellessa, F Marelli, P Duminuco, M Adamo, GE Papadakis, L Bartoloni, N Sato, M Lang-Murit, A Troendle, WS Dhillo, A Morelli, G Guarnieri, N Pitteloud, L Persani, M Bonomi, P Giacobini, V Vezzoli
    JCI Insight, 2023-03-08;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  24. Differential impacts of Cntnap2 heterozygosity and Cntnap2 null homozygosity on axon and myelinated fiber development in mouse
    Authors: Cifuentes-Diaz C, Canali G, Garcia M et al.
    Frontiers in Neuroscience
  25. Deletion of Sphingosine 1-Phosphate receptor 1 in cardiomyocytes during development leads to abnormal ventricular conduction and fibrosis
    Authors: R Jorgensen, M Katta, J Wolfe, DF Leach, B Lavelle, J Chun, LD Wilsbacher
    Physiological Reports, 2021-10-01;9(19):e15060.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  26. NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro
    Authors: A Ribeiro da, EA Neri, LT Turaça, R Dariolli, MH Fonseca-Al, A Santos-Mir, D Roman-Camp, G Venturini, JE Krieger
    Sci Rep, 2020-09-30;10(1):16163.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  27. Afadin Signaling at the Spinal Neuroepithelium Regulates Central Canal Formation and Gait Selection
    Authors: S Skarlatou, C Hérent, E Toscano, CS Mendes, J Bouvier, N Zampieri
    Cell Rep, 2020-06-09;31(10):107741.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Conditional ablation and conditional rescue models for Casq2 elucidate the role of development and of cell type specific expression of Casq2 in the CPVT2 phenotype
    Authors: DJ Flores, T Duong, LO Brandenber, A Mitra, A Shirali, JC Johnson, D Springer, A Noguchi, ZX Yu, SN Ebert, A Ludwig, BC Knollmann, MD Levin, K Pfeifer
    Hum. Mol. Genet., 2018-05-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  29. MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development
    Authors: N Hamada, I Iwamoto, H Tabata, KI Nagata
    Acta Neuropathol Commun, 2017-11-30;5(1):92.
    Species: Primate
    Sample Types: Whole Cells
    Applications: ICC
  30. Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development
    Authors: K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo
    J. Neurosci., 2017-08-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Phenotypically silent Cre recombination within the postnatal ventricular conduction system
    Authors: S Bhattachar, M Bhakta, NV Munshi
    PLoS ONE, 2017-03-30;12(3):e0174517.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  32. NOVA2-mediated RNA regulation is required for axonal pathfinding during development
    Elife, 2016-05-25;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
    Authors: Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido T, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
    EMBO Mol Med, 2015-02-01;7(2):175-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  34. BACE1 activity regulates cell surface contactin-2 levels.
    Authors: Gautam, Vivek, D'Avanzo, Carla, Hebisch, Matthias, Kovacs, Dora M, Kim, Doo Yeon
    Mol Neurodegener, 2014-01-09;9(0):4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons.
    Authors: Kuhn PH, Koroniak K, Hogl S, Colombo A, Zeitschel U, Willem M, Volbracht C, Schepers U, Imhof A, Hoffmeister A, Haass C, Rossner S, Brase S, Lichtenthaler SF
    EMBO J., 2012-06-22;31(14):3157-68.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. Non-cell autonomous control of precerebellar neuron migration by Slit and Robo proteins
    Authors: Chloé Dominici, Quentin Rappeneau, Pavol Zelina, Stéphane Fouquet, Alain Chédotal
    Development
  37. Low-cost multimodal light sheet microscopy for optically cleared tissues and living specimens
    Authors: Vincent Rouger
    J Biomed Opt, 2016-12-01;21(12):126008.
  38. beta -Secretase BACE1 Is Required for Normal Cochlear Function
    Authors: Marlen Dierich, Stephanie Hartmann, Nadine Dietrich, Philip Moeser, Franziska Brede, Lejo Johnson Chacko et al.
    The Journal of Neuroscience
  39. PCP4 regulates Purkinje cell excitability and cardiac rhythmicity
    Authors: Eugene E. Kim, Akshay Shekhar, Jia Lu, Xianming Lin, Fang-Yu Liu, Jie Zhang et al.
    Journal of Clinical Investigation
  40. Transcription factor ETV1 is essential for rapid conduction in the heart
    Authors: Akshay Shekhar, Xianming Lin, Fang-Yu Liu, Jie Zhang, Huan Mo, Lisa Bastarache et al.
    Journal of Clinical Investigation
  41. In vivo visualization and molecular targeting of the cardiac conduction system
    Authors: William R. Goodyer, Benjamin M. Beyersdorf, Lauren Duan, Nynke S. van den Berg, Sruthi Mantri, Francisco X. Galdos et al.
    Journal of Clinical Investigation
  42. Differential impacts of Cntnap2 heterozygosity and Cntnap2 null homozygosity on axon and myelinated fiber development in mouse
    Authors: Cifuentes-Diaz C, Canali G, Garcia M et al.
    Frontiers in Neuroscience
  43. Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function
    Authors: Peer-Hendrik Kuhn, Alessio Vittorio Colombo, Benjamin Schusser, Daniela Dreymueller, Sebastian Wetzel, Ute Schepers et al.
    eLife

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Human/Mouse/Rat Contactin-2/TAG1 Antibody
By Anonymous on 08/05/2020
Application: IHC Sample Tested: mouse heart Species: Mouse
Immunohistochemistry Human/Mouse/Rat Contactin-2/TAG1 Antibody AF4439
Mouse/Rat Contactin‑2/TAG1 Antibody
By Rob Andrew on 04/26/2016
Application: WB Sample Tested: Brain tissue Species: Mouse

80, 40 and 20ug of total mouse brain homogenate was separated by SDS-PAGE and transfered to PVDF membrane
Block: 1% BSA, 1% FSG PBS-T 1h RT
Primary: 1:1000, 1% BSA, 1% FSG PBS-T O/N 4oC
Secondary: 1:5000,1% BSA, 1% FSG PBS-T 2h RT
(Secondary - DaG 800, Licor)

Western Blot Mouse/Rat Contactin‑2/TAG1 Antibody AF4439