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Human/Mouse/Rat Contactin-3 Antibody

Catalog #: AF5539
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5539
AF5539-SP
Detection of Human/Mouse/Rat Contactin‑3 by Western Blot.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Contactin-3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Contactin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Contactin-5 and recombinant mouse Contactin-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Contactin-3
Glu20-Thr1003
Accession # Q07409
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Contactin‑3 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Contactin‑3 by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Contactin-3 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry Contactin-3 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Contactin‑3 in bEnd.3 Mouse Cell Line. Contactin-3 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat Contactin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-3

Contactin-3 (also BIG-1 in rat and PANG in mouse) is a 150 kDa member of the TAG/F3 subgroup, contactin family, Ig superfamily hierarchy. It is a GPI linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. Its exact function is unclear, but it likely mediates cell-cell interaction and may promote neurite outgrowth. Human Contactin-3 preproprecursor is 1028 amino acids (aa) in length. It contains a 19 aa signal sequence, a 983 aa mature region and a 26 aa cleavable C-terminal prosegment. The mature region shows six C2- type IG like domains (aa 26-593), followed by four fibronectin type III modules (aa 598-992). There is one potential alternate start site at Met128. Over aa 26-992, human Contactin-3 shares 92% aa identity with mouse Contactin‑3.

Entrez Gene IDs
5067 (Human); 18488 (Mouse); 54279 (Rat)
Alternate Names
BIG-1; CNTN3; contactin 3 (plasmacytoma associated); Contactin3; Contactin-3; PANG; PCS

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Citations for Human/Mouse/Rat Contactin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Down-regulation of the brain-specific cell-adhesion molecule contactin-3 in tuberous sclerosis complex during the early postnatal period
    Authors: A Korotkov, MJ Luinenburg, A Romagnolo, TS Zimmer, J van Schepp, A Bongaarts, DWM Broekaart, JJ Anink, C Mijnsberge, FE Jansen, W van Hecke, WG Spliet, PC van Rijen, M Feucht, JA Hainfellne, P Krsek, J Zamecnik, PB Crino, K Kotulska, L Lagae, AC Jansen, DJ Kwiatkowsk, S Jozwiak, P Curatolo, A Mühlebner, EA van Vliet, JD Mills, E Aronica
    Journal of neurodevelopmental disorders, 2022-01-15;14(1):8.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Molecular dissection of germline chromothripsis in a developmental context using patient-derived iPS cells
    Authors: Sjors Middelkamp, Sebastiaan van Heesch, A. Koen Braat, Joep de Ligt, Maarten van Iterson, Marieke Simonis et al.
    Genome Medicine

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