Human/Mouse/Rat CrkL Antibody Summary
Met1-Glu303
Accession # P46109
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CrkL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CrkL Monoclonal Antibody (Catalog # MAB5127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CrkL at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse and Rat CrkL by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CrkL Monoclonal Antibody (Catalog # MAB5127) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CrkL at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CrkL
CrkL is a widely-expressed 39 kDa adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and the immune response. Furthermore, this adaptor protein plays an essential role in Chronic Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase.
Product Datasheets
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