Human/Mouse/Rat DYRK2 Antibody

Catalog #: AF5408 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5408
AF5408-SP

Detection of Human/Mouse/Rat DYRK2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat DYRK2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat DYRK2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DYRK2
Met74-Glu180
Accession # Q92630

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat DYRK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, BaF3 mouse pro-B cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat DYRK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5408) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for DYRK2 at approximately 58 to 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DYRK2

DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. DYRK2 is expressed in multiple tissues and shows dual substrate specificity. Autophosphorylation on Tyr382 leads to self-activation, while target proteins are Ser/Thr phosphorylated. Targets include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 by DYRK2 leads to cell apoptosis in response to DNA damage. Human DYRK2 is 601 amino acids (aa) in length, and contains one kinase catalytic domain (aa 222‑535).

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 2

Entrez Gene IDs

8445 (Human); 69181 (Mouse); 314862 (Rat)

Alternate Names

dual specificity tyrosine-phosphorylation-regulated kinase 2; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2; DYRK2; EC 2.7.12; EC 2.7.12.1; FLJ21217; FLJ21365