Human/Mouse/Rat DYRK3 Antibody

Catalog #: MAB5409 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5409
MAB5409-SP

Detection of Human, Mouse, and Rat DYRK3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat DYRK3 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat DYRK3 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 563518

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DYRK3
Asn52-Glu167
Accession # O43781

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat DYRK3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat DYRK3 Monoclonal Antibody (Catalog # MAB5409) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for DYRK3 at approximately 70 kDa and 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DYRK3

DYRK3 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 3; also REDK) is a 70‑72 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and erythroid-lineage precursors, and shows dual substrate specificity; autophosphorylation on Tyr369 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include CREB and Histone 2B. Human DYRK3 is 588 amino acids (aa) in length and contains one kinase catalytic domain (aa 209‑522). There are two potential isoform variants that show either a six aa substitution for aa 1‑26, or this same substitution in combination with a premature truncation after Gln263. DYRK3 immunoreactive proteolysis bands of 30 and 40 kDa have been detected in DYRK3-transfected cells. Over aa 52‑167, human DYRK3 shares 75% aa identity with mouse DYRK3.

Long Name

Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 3

Entrez Gene IDs

8444 (Human); 226419 (Mouse); 304775 (Rat)

Alternate Names

dual specificity tyrosine-phosphorylation-regulated kinase 3; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 5; DYRK3; DYRK5; EC 2.7.12; EC 2.7.12.1; hYAK3-2; protein kinase Dyrk3; REDK; REDKRED; Regulatory erythroid kinase; YAK3-2