Human/Mouse/Rat EMAP-II Antibody

Catalog #: MAB1910 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1910-100
MAB1910-SP

Detection of Human, Mouse, and Rat EMAP‑II by Western Blot.

3 Images

All EMAP-II Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat EMAP-II Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human EMAP-II in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 959516

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human EMAP-II
Ser147-Lys312
Accession # Q12904

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat EMAP‑II by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, SK-OV-3 human ovarian adenocarcinoma cell line, M1 mouse myeloid leukemia cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat EMAP-II Monoclonal Antibody (Catalog # MAB1910) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EMAP-II at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

View Larger

EMAP‑II in HeLa Human Cell Line. EMAP-II was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/ Rat EMAP-II Monoclonal Antibody (Catalog # MAB1910) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

View Larger

EMAP‑II in Human Prostate Cancer Tissue. EMAP-II was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human/Mouse/Rat EMAP-II Monoclonal Antibody (Catalog # MAB1910) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMAP-II

EMAP-II is a pro-inflammatory cytokine that is chemotactic for monocytes and granulocytes. It is a cleavage product of multisynthetase complex auxiliary component p43.

Long Name

Endothelial-Monocyte Activating Polypeptide II

Entrez Gene IDs

9255 (Human); 13722 (Mouse); 114632 (Rat)

Alternate Names

AIMP1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; EMAP-2; EMAPII; EMAP-II; EMAPIImember 1 (endothelialmonocyte-activating); endothelial-monocyte activating polypeptide II; Multisynthase complex auxiliary component p43; multisynthetase complex auxiliary component p43; SCYE1