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Human/Mouse/Rat ERK1/ERK2 Antibody

Catalog #: MAB1576
20 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1576
MAB1576-SP
Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot.
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ERK1/ERK2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat ERK1/ERK2 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 216703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ERK1
Met1-Pro379
Accession # P27361
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat ERK1/ERK2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Immunohistochemistry ERK1/ERK2 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

ERK1/ERK2 in Human Brain. ERK1/ERK2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. The two proteins share 83% amino acid identity, differing mainly at the N and C termini.

Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1; ERK-1; ERK1/ERK2; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; p44erk1; p44-ERK1; p44mapk; p44-MAPK; PRKM3

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Citations for Human/Mouse/Rat ERK1/ERK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. IGF1R Is a Potential New Therapeutic Target for HGNET-BCOR Brain Tumor Patients
    Authors: N Vewinger, S Huprich, L Seidmann, A Russo, F Alt, H Bender, C Sommer, D Samuel, N Lehmann, N Backes, L Roth, PN Harter, K Filipski, J Faber, C Paret
    Int J Mol Sci, 2019-06-21;20(12):.
  2. Oxytocin Receptors on Calvarial Periosteal Innervation: Therapeutic Target for Post-Traumatic Headache?
    Authors: Bharadwaj, VN;Klukinov, M;Cowan, RP;Mahinparvar, N;Clark, DJ;Yeomans, DC;
    Pharmaceutics
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury
    Authors: Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li
    iScience
  4. Chrysanthemum boreale Makino Inhibits Oxidative Stress-Induced Neuronal Damage in Human Neuroblastoma SH-SY5Y Cells by Suppressing MAPK-Regulated Apoptosis
    Authors: Parkyong Song, Seo Young Choi, Ji Sun Hwang, Hyeon Cheal Park, Keun Ki Kim, Hong-Joo Son et al.
    Molecules
  5. Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis
    Authors: M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk
    Oncogene, 2022-06-14;0(0):.
    Species: Xenograft
    Sample Types: Tissue Homogenates
    Applications: Simple Western
  6. Clusterin and Related Scoring Index as Potential Early Predictors of Response to Sorafenib in Hepatocellular Carcinoma
    Authors: Satoshi Narahara, Takehisa Watanabe, Katsuya Nagaoka, Nahoko Fujimoto, Yoki Furuta, Kentaro Tanaka et al.
    Hepatology Communications
  7. Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning
    Authors: E Soleimanpo, JR Bergado Ac, P Landgraf, D Mayer, E Dankert, DC Dieterich, M Fendt
    Cells, 2021-01-26;10(2):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Safety and Activity of the Combination of Ceritinib and Dasatinib in Osteosarcoma
    Authors: O Beck, C Paret, A Russo, J Burhenne, M Fresnais, K Steimel, L Seidmann, DC Wagner, N Vewinger, N Lehmann, M Sprang, N Backes, L Roth, MA Neu, A Wingerter, N Henninger, KE Malki, H Otto, F Alt, A Desuki, T Kindler, J Faber
    Cancers (Basel), 2020-03-26;12(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  9. Immunodeficiency in a Patient with 22q11.2 Distal Deletion Syndrome and a p.Ala7dup Variant in the MAPK1 Gene
    Authors: Ana I. Sánchez, Mary A. García-Acero, Angela Paredes, Rossi Quero, Rita I. Ortega, Jorge A. Rojas et al.
    Molecular Syndromology
  10. mTOR-dependent phosphorylation controls TFEB nuclear export
    Authors: Gennaro Napolitano, Alessandra Esposito, Heejun Choi, Maria Matarese, Valerio Benedetti, Chiara Di Malta et al.
    Nature Communications
  11. Chronic irradiation with low-dose-rate 137Cs-gamma rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation
    Authors: Katoh Shinsuke, Kobayashi Junya, Umeda Tomonobu, Kobayashi Yoshiko, Nobuo Izumo, Suzuki Takahiko
    Journal of Radiation Research
  12. Wilms' tumor gene 1 silencing inhibits proliferation of human osteosarcoma MG-63 cell line by cell cycle arrest and apoptosis activation
    Authors: Adriana Carol Eleonora Graziano, Venera Cardile, Rosanna Avola, Nunzio Vicario, Carmela Parenti, Lucia Salvatorelli et al.
    Oncotarget
  13. Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells
    J Biol Chem, 2016-02-24;291(19):10277-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Extremely low frequency electromagnetic fields affect proliferation and mitochondrial activity of human cancer cell lines
    Authors: Michele Destefanis, Marta Viano, Christian Leo, Gianpiero Gervino, Antonio Ponzetto, Francesca Silvagno
    International Journal of Radiation Biology
  15. The Proinflammatory Cytokine High-Mobility Group Box-1 Mediates Retinal Neuropathy Induced by Diabetes
    Authors: Ahmed M. Abu El-Asrar, Mohammad Mairaj Mairaj Siddiquei, Mohd Imtiaz Nawaz, Karel Geboes, Ghulam Mohammad
    Mediators of Inflammation
  16. The carotenoid lutein enhances matrix metalloproteinase-9 production and phagocytosis through intracellular ROS generation and ERK1/2, p38 MAPK, and RARbeta activation in murine macrophages.
    Authors: Lo H, Chen C, Yang C, Wu P, Tsou C, Chiang K, Wu W
    J Leukoc Biol, 2013-02-19;93(5):723-35.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways.
    Authors: Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G
    PLoS ONE, 2012-09-17;7(9):e45257.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages.
    Authors: Lane AE, Tan JT, Hawkins CL
    Biochem. J., 2010-08-15;430(1):161-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010-01-06;48(6):1225-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients
    Respir. Res., 2016-11-08;17(1):145.

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Reviews for Human/Mouse/Rat ERK1/ERK2 Antibody

Average Rating: 4 (Based on 5 Reviews)

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Human/Mouse/Rat ERK1/ERK2 Antibody
By Anonymous on 09/08/2021
Application: IHC Sample Tested: Mammary tissue Species: Human
Immunohistochemistry Human/Mouse/Rat ERK1/ERK2 Antibody MAB1576
Human/Mouse/Rat ERK1/ERK2 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human/Mouse/Rat ERK1/ERK2 Antibody MAB1576
Human/Mouse/Rat ERK1/ERK2 Antibody
By Carly Garrison on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human/Mouse/Rat ERK1/ERK2 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human/Mouse/Rat ERK1/ERK2 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human