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Human/Mouse/Rat Erythropoietin/EPO Antibody

Catalog #: AF959
7 Citations Datasheet / COA / SDS
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AF959
AF959-SP
Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin/EPO Antibody.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Erythropoietin/EPO Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Erythropoietin/EPO in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Thymopoietin (Tpo) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin/EPO
Ala27-Arg192
Accession # P07321
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Erythropoietin/EPO (Ultrapure) (Catalog # 286-EP)
Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME)
Recombinant Rat Erythropoietin/EPO (Catalog # 1306-RE)
Neutralization
Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 10 ng/mL Recombinant Mouse Erythropoietin/EPO.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin/EPO Antibody. View Larger

Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin/EPO Antibody. Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Erythropoietin/EPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF959). The ND50 is typically 0.25-1.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of EPO is required for biological activities in vivo (4). Mature mouse EPO shares 95% amino acid sequence identity with rat EPO and 73%-82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

References
  1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
  2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.
Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

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Citations for Human/Mouse/Rat Erythropoietin/EPO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors
    Authors: Hosaka K, Wang C, Zhang S et al.
    Cancer communications (London, England)
  2. Human iPSC-derived neural crest stem cells can produce EPO and induce erythropoiesis in anemic mice
    Authors: V Brizi, S Buttò, D Cerullo, A Michele La, R Rodrigues-, R Novelli, D Corna, A Benigni, G Remuzzi, C Xinaris
    Stem Cell Research, 2021-07-25;55(0):102476.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions
    Authors: K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama
    J Neuroinflammation, 2020-05-02;17(1):141.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Venlafaxine Mitigates Depressive-Like Behavior in Ovariectomized Rats by Activating the EPO/EPOR/JAK2 Signaling Pathway and Increasing the Serum Estradiol Level
    Authors: Muhammed A. Saad, Ayman E. El-Sahar, Rabab H. Sayed, Eman M. Elbaz, Hebatullah S. Helmy, Mahmoud A. Senousy
    Neurotherapeutics
  5. Zinc Supplementation Stimulates Red Blood Cell Formation in Rats
    Authors: YH Chen, HL Feng, SS Jeng
    Int J Mol Sci, 2018-09-18;19(9):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  6. In vivo intermittent hypoxia elicits enhanced expansion and neuronal differentiation in cultured neural progenitors
    Authors: Heather H. Ross, Milap S. Sandhu, Tina F. Cheung, Garrett M. Fitzpatrick, Warren J. Sher, Alexander J. Tiemeier et al.
    Experimental Neurology
  7. Suppression of hepcidin during anemia requires erythropoietic activity.
    Authors: Pak M, Lopez MA, Gabayan V, Ganz T, Rivera S
    Blood, 2006-08-01;108(12):3730-5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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