Human/Mouse/Rat FABP1/L-FABP Antibody Summary
Met1-Ile127
Accession # P02692
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot. Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line, mouse liver tissue, human liver tissue, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/ Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Rat FABP1/L‑FABP by Western Blot. Western blot shows lysate of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/ Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/ L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FABP1 in Rat Liver. FABP1 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Human/Mouse/Rat FABP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of Mouse and Rat FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of H4-II-E-C3 rat hepatoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 16 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP1/L-FABP
FABP1, also known as liver FABP (L-FABP, Z-protein and squalene, and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney, and lung. FABP1 binds free fatty acids, their co-enzyme A derivatives, and may be involved in intracellular lipid transport.
Product Datasheets
Citations for Human/Mouse/Rat FABP1/L-FABP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Expression and enhancement of FABP4 in septoclasts of the growth plate in FABP5-deficient mouse tibiae
Authors: Yasuhiko Bando, Nobuko Tokuda, Yudai Ogasawara, Go Onozawa, Arata Nagasaka, Koji Sakiyama et al.
Histochemistry and Cell Biology
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Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo
Authors: Lorenzo-Martín, LF;Hübscher, T;Bowler, AD;Broguiere, N;Langer, J;Tillard, L;Nikolaev, M;Radtke, F;Lutolf, MP;
Nature
Species: Transgenic Mouse
Sample Types: Organoid
Applications: Immunohistochemistry -
Identification of biomarkers associated with the development of hepatocellular carcinoma in CuZn superoxide dismutase deficient mice.
Authors: Elchuri S, Naeemuddin M, Sharpe O, Robinson WH, Huang TT
Proteomics, 2007-06-01;7(12):2121-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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