Human/Mouse/Rat FANCD2 Antibody Summary
Glu11-Glu230
Accession # Q9BXW9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat FANCD2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat FANCD2 Monoclonal Antibody (Catalog # MAB93691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human FANCD2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat FANCD2 Monoclonal Antibody (Catalog # MAB93691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FANCD2
Fanconi anemia group D2 protein, encoded by the FANCD2 gene, is a member of the Fanconi anemia complementation (FANC) group that also includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCE, FANCF, FANCG, and FANCL. The members of the Fanconi anemia complementation group share little sequence similarities.
Product Datasheets
Citation for Human/Mouse/Rat FANCD2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Clonal hematopoiesis driven by chromosome 1q/MDM4 trisomy defines a canonical route toward leukemia in Fanconi anemia
Authors: M Sebert, S Gachet, T Leblanc, A Rousseau, O Bluteau, R Kim, R Ben Abdela, F Sicre de F, L Maillard, C Fedronie, V Murigneux, L Bellenger, N Naouar, S Quentin, L Hernandez, N Vasquez, M Da Costa, PH Prata, L Larcher, M de Tersant, M Duchmann, A Raimbault, F Trimoreau, O Fenneteau, W Cuccuini, N Gachard, N Auger, G Tueur, M Blanluet, C Gazin, M Souyri, F Langa Vive, A Mendez-Ber, H Lapillonne, E Lengline, E Raffoux, P Fenaux, L Adès, E Forcade, C Jubert, C Domenech, M Strullu, B Bruno, N Buchbinder, C Thomas, A Petit, G Leverger, G Michel, M Cavazzana, E Gluckman, Y Bertrand, N Boissel, A Baruchel, JH Dalle, E Clappier, E Gilson, L Deriano, S Chevret, F Sigaux, G Socié, D Stoppa-Lyo, H de Thé, C Antoniewsk, D Bluteau, R Peffault d, J Soulier
Cell Stem Cell, 2023-02-02;30(2):153-170.e9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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