Human/Mouse/Rat Fibromodulin/FMOD Antibody

Catalog # Availability Size / Price Qty
AF5945
AF5945-SP

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Detection of Human/Mouse/Rat Fibromodulin by Western Blot.
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Citations (1)
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Human/Mouse/Rat Fibromodulin/FMOD Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Fibromodulin/FMOD in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fibromodulin/FMOD
Asp75-Ile376
Accession # Q06828
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.3 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Fibromodulin antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Fibromodulin by Western Blot. Western blot shows lysates of human cartilage, human ovarian cancer, and rat uterine tissue. PVDF membrane was probed with 0.3 µg/mL of Sheep Anti-Human/Mouse/Rat Fibromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5945) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibromodulin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Detection of Zebrafish Fibromodulin/FMOD by Immunohistochemistry View Larger

Detection of Zebrafish Fibromodulin/FMOD by Immunohistochemistry SLRPs are enriched in human spinal cord lesions.Anti-LUM, anti-PRELP, anti-CHAD, and anti-FMOD immunoreactivity is increased (arrowheads; see calibration bar of lookup table (LUT)) in the epicenter of the injured human spinal cord at nine days-post injury, as compared to rostral control segments of the same patient. Note that immunoreactivity is mainly observed in proximity to the hemorrhage (black; see calibration bar of LUT). Also note that with the exception of blood-derived autofluorescence, individual channels show distinct pattern of immunofluorescence (IF) signal (insets). Shown are transversal sections (dorsal is up). Similar results were obtained for anti-LUM in five out of six cases, for anti-PRELP and anti-FMOD in four out of six cases, and anti-CHAD in two out of six cases (Supplementary Table 3). Scale bars: 2 mm, 200 µm (insets). The human icon in the panel was created using BioRender. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37884489), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibromodulin/FMOD

Fibromodulin (FMOD; also FM and collagen-binding 59 kDa protein) is a secreted, 60‑70 kDa class II member of the SLRP family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-beta. Fibromodulin is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers. Mature human Fibromodulin is 358 amino acids (aa) in length (aa 19‑376). It contains 12 LRRs (Leu-rich repeats) (aa 98‑376), with the seventh and eleventh LRR principally responsible for collagen type I binding. Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation. There is one splice form that shows a deletion of aa 88‑101. Over aa 76‑376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin.

Entrez Gene IDs
2331 (Human); 14264 (Mouse); 64507 (Rat)
Alternate Names
Collagen-binding 59 kDa protein; fibromodulin proteoglycan; Fibromodulin; FM; FMOD; Keratan sulfate proteoglycan fibromodulin; SLRR2E; SLRR2EKSPG fibromodulin

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Citation for Human/Mouse/Rat Fibromodulin/FMOD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment
    Authors: Kolb, J;Tsata, V;John, N;Kim, K;Möckel, C;Rosso, G;Kurbel, V;Parmar, A;Sharma, G;Karandasheva, K;Abuhattum, S;Lyraki, O;Beck, T;Müller, P;Schlü beta ler, R;Frischknecht, R;Wehner, A;Krombholz, N;Steigenberger, B;Beis, D;Takeoka, A;Blümcke, I;Möllmert, S;Singh, K;Guck, J;Kobow, K;Wehner, D;
    Nature communications
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry

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