Human/Mouse/Rat FKBP13 Antibody

Catalog #: AF4356 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4356
AF4356-SP

Detection of Human/Mouse/Rat FKBP13 by Western Blot.

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Human/Mouse/Rat FKBP13 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat FKBP13 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human FKBP12, FKBP25, FKBP38, FKBP51 or FKBP52.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FKBP13
Ala22-Leu142
Accession # P26885

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat FKBP13 by Western Blot. Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP13

FK506 binding protein, 13 kilodalton molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑proline residues critical for proper folding of proteins. The immunosuppressants FK506 and rapamycin are potent inhibitors of FKBP13. This isomerase is localized in the endoplasmic reticulum and is also associated with cytoskeletal elements such as erythrocyte band 4.1. FKBP13 levels are increased by treatments that affect golgi protein processing such as tunicamycin and heat shock, suggesting that FKBP13 is important in the unfolded protein response observed with endoplasmic reticulum stress.

Long Name

13 kDa FK506 Binding Protein

Entrez Gene IDs

2286 (Human); 14227 (Mouse); 25639 (Rat)

Alternate Names

EC 5.2.1.8,13 kDa FK506-binding protein; FK506 binding protein 2, 13kDa; FK506-binding protein 2 (13kD); FK506-binding protein 2; FKBP13; FKBP13,13 kDa FKBP; FKBP-13proline isomerase; FKBP2; FKBP-2; Immunophilin FKBP13; peptidyl-prolyl cis-trans isomerase FKBP2; PPIase FKBP2; PPIase; rapamycin-binding protein; rotamase