Home / FKBP38 / Human/Mouse/Rat FKBP38 Antibody

Human/Mouse/Rat FKBP38 Antibody

Catalog #: MAB3580
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3580
MAB3580-SP
Detection of Human/Mouse/Rat FKBP38 by Western Blot.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FKBP38 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat FKBP38 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 391505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FKBP38
Met58-Gly382
Accession # Q14318
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat FKBP38 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat FKBP38 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat FKBP38 Monoclonal Antibody (Catalog # MAB3580) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FKBP38 at approximately 60 to 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP38

FK506 Binding Proteins (FKBPs) are intracellular receptors for the immuno-suppressive drug FK506. The FKBP/FK506 complex exerts its immunosuppressive effects by inhibiting calcineurin, a calcium- and calmodulin-dependent serine/threonine phosphatase that functions as a critical signaling molecule during T-cell activation. FKBP38, also known as FKBP8, is a 355 to 412 amino acid (aa) protein with an apparent molecular mass of ~60‑64 kDa in SDS-PAGE. FKBP38 binds to and inhibits calcineurin even in the absence of FK506, indicating that FKBP38 is a constitutively active inhibitor of calcineurin. Additionally, FKBP38 co‑immunoprecipitates with Bcl-2 and Bcl-xL, suggesting that FKBP38 may regulate apoptosis by anchoring Bcl‑2 and Bcl‑xL to mitochondrial membranes. Mouse FKBP38 shares 94% and 87% sequence identity with human and rat FKBP38, respectively.

Long Name
38 kDa FK506 Binding Protein
Entrez Gene IDs
23770 (Human); 14232 (Mouse); 290652 (Rat)
Alternate Names
38 kDa FK506-binding protein; EC 5.2.1.8,38 kDa FKBP; FK506 binding protein 8, 38kDa; FK506-binding protein 8 (38kD); FK506-binding protein 8; FKBP38; FKBP-38; FKBP38PPIase FKBP8; FKBP8; FKBP-8; FKBPr38; hFKBP38; peptidyl-prolyl cis-trans isomerase FKBP8; rotamase

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Citations for Human/Mouse/Rat FKBP38 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Macrocycles that inhibit the binding between heat shock protein 90 and TPR-containing proteins.
    Authors: Ardi V, Alexander LD, Johnson VA et al.
    ACS Chem Biol
  2. Adding Fingers to an Engineered Zinc Finger Nuclease Can Reduce Activity.
    Authors: Shimizu Y, Sollu C, Meckler JF et al.
    Biochemistry;50(22):5033-5041.
  3. ZMYND10 functions in a chaperone relay during axonemal dynein assembly.
    Authors: Mali GR, YeYati PL, Mizuno S et al.
    Elife.
  4. Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery.
    Authors: Bobis-Wozowicz S, Galla M, Alzubi J et al.
    Sci Rep
  5. Mitophagy mediated by BNIP3 and NIX protects against ferroptosis by downregulating mitochondrial reactive oxygen species
    Authors: Yamashita, SI;Sugiura, Y;Matsuoka, Y;Maeda, R;Inoue, K;Furukawa, K;Fukuda, T;Chan, DC;Kanki, T;
    Cell death and differentiation
    Species: Human
    Sample Types:
    Applications: Western Blot
  6. Exchange protein directly activated by cAMP 2 is required for corticotropin‐releasing hormone‐mediated spine loss
    Authors: Zhong Xie, Peter Penzes, Deepak P. Srivastava
    European Journal of Neuroscience
  7. A Serotonin Circuit Acts as an Environmental Sensor to Mediate Midline Axon Crossing through EphrinB2
    Authors: Lingyan Xing, Jong-Hyun Son, Tamara J. Stevenson, Christina Lillesaar, Laure Bally-Cuif, Tiffanie Dahl et al.
    The Journal of Neuroscience
  8. Phosphatidic Acid Activates Mammalian Target of Rapamycin Complex 1 (mTORC1) Kinase by Displacing FK506 Binding Protein 38 (FKBP38) and Exerting an Allosteric Effect.
    Authors: Yoon MS, Sun Y, Arauz E
    J. Biol. Chem., 2011-07-07;286(34):29568-74.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. A novel zinc-finger nuclease platform with a sequence-specific cleavage module.
    Authors: Schierling B, Dannemann N, Gabsalilow L et al.
    Nucleic Acids Research.
  10. Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases.
    Authors: Osiak A, Radecke F, Guhl E et al.
    PLoS ONE 6(12).

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