Human/Mouse/Rat FPRP/PTGFRN Antibody

Detection of Human, Mouse, and Rat FPRP/PTGFRN by Western Blot. Western blot shows lysates of LoVo human colorectal adenocarcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB10043) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FPRP at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FPRP/PTGFRN in Human Lung Cancer Tissue. FPRP/PTGFRN was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB10043) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.