Human/Mouse/Rat GAPDH Antibody Summary
Met1-Ala150
Accession # P04406
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAPDH/G3PDH at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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GAPDH in HeLa Human Cell Line. GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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GAPDH/G3PDH in Human Kidney. GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.
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GAPDH in Human Prostate Cancer Tissue. GAPDH was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human GAPDH by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Mouse GAPDH by Western Blot Role of Akt pathway in aliskiren's effects on attenuating collagen expression.A and B demonstrate the results of Western blotting for phospho- and total Akt in hearts of mice treated with various diets. C shows changes in the protein levels of collagen type I, phospho- and total Akt in response to 24 hours of exposure to D,L. Homocysteine (200 µmol/L) with or without pre-treatment with aliskiren 10 µmol/L (AL), wortmannin 1 µmol/L or Akt Inhibitor VIII 1 µmol/L. Hhe – Hhe-inducing diet; D,L, homocysteine (lower panel); AL 0.05– aliskiren 0.5 mg/kg/day; AL 5– aliskiren 5 mg/kg/day; AL 50– aliskiren 50 mg/kg/day; Wort – wortmannin; Akt in – Akt inhibitor VIII. *p<0.05; **p<0.01; and ***p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24349097), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Rat GAPDH by Western Blot Effect of prodigiosins on the mRNA and protein expression of HO-1 in gastric tissue.Data (means ± SEM of three assays) were normalized to the levels of GAPDH and expressed as fold induction (log2 scale), relative to the level of HO-1 in the control. aP < 0.05, significant difference vs. the health rats; bP < 0.05, significant difference vs. HCl/ethanol-induced rats, Duncan's post-hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31194753), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human GAPDH by Western Blot The DU145FP cells express higher levels of cancer stemness related genes. (A) Transcriptome analyses of prostate cancer stemness-related markers. The Log2 transformed ratio of FPKM values (e.g., FP/WT) are indicated by color-coded index bars. (B) Cells were stained with fluorophore conjugated monoclonal antibodies against CD44 and EPCAM, the antibody against integrin alpha 2 beta 3 without fluorophore conjugation was detected by using secondary antibody conjugated with fluorophore, and stained cells were analyzed by flow cytometry. The scatter plots and histograms show representative results. CTRL denote cell stained with isotype control antibody or only secondary antibody; ST denote cell stained with antibody against EPCAM and integrin alpha 2 beta 3; US denote unstained cells. (C) Immunoblotting confirmation of the protein expression of A-tubulin, GAPDH, SOX9, SLUG, HIF1A, HIF2A, CD44 and integrin alpha 2 beta 3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28698547), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Rat GAPDH by Western Blot Effects of aliskiren on homocysteine-induced matrix expression in cultured cardiac fibroblasts.Fibroblasts were cultured in the presence or absence of D,L, homocysteine 200 µmol/L for 24 hours after pretreatment with varying concentrations of aliskiren (0.1–10 µmol/L). Real time PCR was done to measure relative expression of COLIA2 (alpha 2 chain of type I collagen; A) and COL3A1 (alpha 1 chain of type III collagen; B) genes. Figure 5C demonstrates the results of real time PCR to compare the effects of losartan and captopril with aliskiren on homocysteine induced expression of COL1A2 gene. Western blotting was conducted to measure the expression of collagen type I protein (D and E) Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) was used as internal control. Hcy-D,L, homocysteine; AL-aliskiren; Los – losartan; and Cap – captopril. *p<0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24349097), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse GAPDH by Western Blot Role of Akt pathway in aliskiren's effects on attenuating collagen expression.A and B demonstrate the results of Western blotting for phospho- and total Akt in hearts of mice treated with various diets. C shows changes in the protein levels of collagen type I, phospho- and total Akt in response to 24 hours of exposure to D,L. Homocysteine (200 µmol/L) with or without pre-treatment with aliskiren 10 µmol/L (AL), wortmannin 1 µmol/L or Akt Inhibitor VIII 1 µmol/L. Hhe – Hhe-inducing diet; D,L, homocysteine (lower panel); AL 0.05– aliskiren 0.5 mg/kg/day; AL 5– aliskiren 5 mg/kg/day; AL 50– aliskiren 50 mg/kg/day; Wort – wortmannin; Akt in – Akt inhibitor VIII. *p<0.05; **p<0.01; and ***p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24349097), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse GAPDH by Western Blot Effects of aliskiren on myocardial matrix gene expression.Real time PCR was performed with ventricular tissue obtained from mice after 12-inducing diet and different doses of aliskiren. Figure 3A demonstrates changes in expression of COL1A1(alpha 1 chain of type I collagen) and 3B shows changes in COL1A2(alpha2 chain of type I collagen) genes. Lower right panels (3C and 3D) demonstrate the results of western blotting for collagen type I in various groups. Hhe – Hhe-inducing diet; AL 0.5– aliskiren 0.5 mg/kg/day; AL 5– aliskiren 5 mg/kg/day; and AL 50– aliskiren 50 mg/kg/day. * p<0.05; and *** p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24349097), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium evades autophagy by disrupting Sirt1-dependent AMPK activation.(A) Immunofluorescence image of S. Typhimurium co-localization with LC3 in GFP-LC3 expressing BMDMs at indicated time points. Data shown are representative of 3 independent experiments (n = 3). (B) Immunoblot analysis of p62 and LC3 expression upon S. Typhimurium infection in BMDMs. (C) LC3 and p62 expression levels are quantified by densitometry analysis. Data shown are from 3 independent experiments. (D) Confocal image of macrophages stained for Sirt1 and LC3. (E) BMDMs stained for LC3 and AMPK upon S. Typhimurium infection. (F) Confocal image of macrophages stained for LKB1 and LC3. (G) Immunoblot analysis of Sirt1, AMPK and LKB1 in wild type (WT) and Atg7-deficient macrophages. (H) Densitometric analysis of Sirt1, AMPK and LKB1 immunoblots (n = 3). Bar graphs are expressed as mean ± SEM, ***p≤0.001, **p≤0.01 and *p≤0.05. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium mediated targeting of Sirt1 for lysosomal degradation is virulence dependent.Immunoblot analysis of ACC and LKB1 activation upon infection with delta ssrB(A) and delta ssaV(B) compared to S. Typhimurium. Sirt1 and acetylated-NF kappa B from macrophages infected with delta ssrB(C)and delta ssaV(D) compared to S. Typhimurium. (E) Expression of Sirt1 from cytoplasmic (C) and nuclear (N) fraction from BMDMs infected with delta ssrB. (F) Confocal image of Sirt1 and LysoTracker Red in delta ssrB-infected BMDMs. Sirt1-LysoTracker Red co-localization in untreated BMDMs infected with S. Typhimurium for 4h is shown for comparison (n = 3). (G) Immunoblot analysis of LC3 and p62. (H) Densitometric analysis of LC3 lipidation and p62 (n = 4). (I) Immunofluorescence image of delta ssrB-infected BMDMs stained for LC3 and LPS of S. Typhimurium (n = 3). (J) Quantitation of LC3 co-localization with SCVs. 100 SCVs were counted and expressed as percentage co-localization. (K) AKT, mTOR, p70S6K, NDRG1 expression upon S. Typhimurium (ST) and delta ssrB infection in BMDMs. (L) Densitometric analysis of AKT, mTOR, p70S6K and NDRG1 are shown from 3 independent experiments. Bar graphs are expressed as mean ± SEM, ***p≤0.001. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium evades autophagy by disrupting Sirt1-dependent AMPK activation.(A) Immunofluorescence image of S. Typhimurium co-localization with LC3 in GFP-LC3 expressing BMDMs at indicated time points. Data shown are representative of 3 independent experiments (n = 3). (B) Immunoblot analysis of p62 and LC3 expression upon S. Typhimurium infection in BMDMs. (C) LC3 and p62 expression levels are quantified by densitometry analysis. Data shown are from 3 independent experiments. (D) Confocal image of macrophages stained for Sirt1 and LC3. (E) BMDMs stained for LC3 and AMPK upon S. Typhimurium infection. (F) Confocal image of macrophages stained for LKB1 and LC3. (G) Immunoblot analysis of Sirt1, AMPK and LKB1 in wild type (WT) and Atg7-deficient macrophages. (H) Densitometric analysis of Sirt1, AMPK and LKB1 immunoblots (n = 3). Bar graphs are expressed as mean ± SEM, ***p≤0.001, **p≤0.01 and *p≤0.05. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium mediated targeting of Sirt1 for lysosomal degradation is virulence dependent.Immunoblot analysis of ACC and LKB1 activation upon infection with delta ssrB(A) and delta ssaV(B) compared to S. Typhimurium. Sirt1 and acetylated-NF kappa B from macrophages infected with delta ssrB(C)and delta ssaV(D) compared to S. Typhimurium. (E) Expression of Sirt1 from cytoplasmic (C) and nuclear (N) fraction from BMDMs infected with delta ssrB. (F) Confocal image of Sirt1 and LysoTracker Red in delta ssrB-infected BMDMs. Sirt1-LysoTracker Red co-localization in untreated BMDMs infected with S. Typhimurium for 4h is shown for comparison (n = 3). (G) Immunoblot analysis of LC3 and p62. (H) Densitometric analysis of LC3 lipidation and p62 (n = 4). (I) Immunofluorescence image of delta ssrB-infected BMDMs stained for LC3 and LPS of S. Typhimurium (n = 3). (J) Quantitation of LC3 co-localization with SCVs. 100 SCVs were counted and expressed as percentage co-localization. (K) AKT, mTOR, p70S6K, NDRG1 expression upon S. Typhimurium (ST) and delta ssrB infection in BMDMs. (L) Densitometric analysis of AKT, mTOR, p70S6K and NDRG1 are shown from 3 independent experiments. Bar graphs are expressed as mean ± SEM, ***p≤0.001. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium mediated targeting of Sirt1 for lysosomal degradation is virulence dependent.Immunoblot analysis of ACC and LKB1 activation upon infection with delta ssrB(A) and delta ssaV(B) compared to S. Typhimurium. Sirt1 and acetylated-NF kappa B from macrophages infected with delta ssrB(C)and delta ssaV(D) compared to S. Typhimurium. (E) Expression of Sirt1 from cytoplasmic (C) and nuclear (N) fraction from BMDMs infected with delta ssrB. (F) Confocal image of Sirt1 and LysoTracker Red in delta ssrB-infected BMDMs. Sirt1-LysoTracker Red co-localization in untreated BMDMs infected with S. Typhimurium for 4h is shown for comparison (n = 3). (G) Immunoblot analysis of LC3 and p62. (H) Densitometric analysis of LC3 lipidation and p62 (n = 4). (I) Immunofluorescence image of delta ssrB-infected BMDMs stained for LC3 and LPS of S. Typhimurium (n = 3). (J) Quantitation of LC3 co-localization with SCVs. 100 SCVs were counted and expressed as percentage co-localization. (K) AKT, mTOR, p70S6K, NDRG1 expression upon S. Typhimurium (ST) and delta ssrB infection in BMDMs. (L) Densitometric analysis of AKT, mTOR, p70S6K and NDRG1 are shown from 3 independent experiments. Bar graphs are expressed as mean ± SEM, ***p≤0.001. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium mediated targeting of Sirt1 for lysosomal degradation is virulence dependent.Immunoblot analysis of ACC and LKB1 activation upon infection with delta ssrB(A) and delta ssaV(B) compared to S. Typhimurium. Sirt1 and acetylated-NF kappa B from macrophages infected with delta ssrB(C)and delta ssaV(D) compared to S. Typhimurium. (E) Expression of Sirt1 from cytoplasmic (C) and nuclear (N) fraction from BMDMs infected with delta ssrB. (F) Confocal image of Sirt1 and LysoTracker Red in delta ssrB-infected BMDMs. Sirt1-LysoTracker Red co-localization in untreated BMDMs infected with S. Typhimurium for 4h is shown for comparison (n = 3). (G) Immunoblot analysis of LC3 and p62. (H) Densitometric analysis of LC3 lipidation and p62 (n = 4). (I) Immunofluorescence image of delta ssrB-infected BMDMs stained for LC3 and LPS of S. Typhimurium (n = 3). (J) Quantitation of LC3 co-localization with SCVs. 100 SCVs were counted and expressed as percentage co-localization. (K) AKT, mTOR, p70S6K, NDRG1 expression upon S. Typhimurium (ST) and delta ssrB infection in BMDMs. (L) Densitometric analysis of AKT, mTOR, p70S6K and NDRG1 are shown from 3 independent experiments. Bar graphs are expressed as mean ± SEM, ***p≤0.001. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium evades autophagy by disrupting Sirt1-dependent AMPK activation.(A) Immunofluorescence image of S. Typhimurium co-localization with LC3 in GFP-LC3 expressing BMDMs at indicated time points. Data shown are representative of 3 independent experiments (n = 3). (B) Immunoblot analysis of p62 and LC3 expression upon S. Typhimurium infection in BMDMs. (C) LC3 and p62 expression levels are quantified by densitometry analysis. Data shown are from 3 independent experiments. (D) Confocal image of macrophages stained for Sirt1 and LC3. (E) BMDMs stained for LC3 and AMPK upon S. Typhimurium infection. (F) Confocal image of macrophages stained for LKB1 and LC3. (G) Immunoblot analysis of Sirt1, AMPK and LKB1 in wild type (WT) and Atg7-deficient macrophages. (H) Densitometric analysis of Sirt1, AMPK and LKB1 immunoblots (n = 3). Bar graphs are expressed as mean ± SEM, ***p≤0.001, **p≤0.01 and *p≤0.05. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium mediated targeting of Sirt1 for lysosomal degradation is virulence dependent.Immunoblot analysis of ACC and LKB1 activation upon infection with delta ssrB(A) and delta ssaV(B) compared to S. Typhimurium. Sirt1 and acetylated-NF kappa B from macrophages infected with delta ssrB(C)and delta ssaV(D) compared to S. Typhimurium. (E) Expression of Sirt1 from cytoplasmic (C) and nuclear (N) fraction from BMDMs infected with delta ssrB. (F) Confocal image of Sirt1 and LysoTracker Red in delta ssrB-infected BMDMs. Sirt1-LysoTracker Red co-localization in untreated BMDMs infected with S. Typhimurium for 4h is shown for comparison (n = 3). (G) Immunoblot analysis of LC3 and p62. (H) Densitometric analysis of LC3 lipidation and p62 (n = 4). (I) Immunofluorescence image of delta ssrB-infected BMDMs stained for LC3 and LPS of S. Typhimurium (n = 3). (J) Quantitation of LC3 co-localization with SCVs. 100 SCVs were counted and expressed as percentage co-localization. (K) AKT, mTOR, p70S6K, NDRG1 expression upon S. Typhimurium (ST) and delta ssrB infection in BMDMs. (L) Densitometric analysis of AKT, mTOR, p70S6K and NDRG1 are shown from 3 independent experiments. Bar graphs are expressed as mean ± SEM, ***p≤0.001. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium mediated targeting of Sirt1 for lysosomal degradation is virulence dependent.Immunoblot analysis of ACC and LKB1 activation upon infection with delta ssrB(A) and delta ssaV(B) compared to S. Typhimurium. Sirt1 and acetylated-NF kappa B from macrophages infected with delta ssrB(C)and delta ssaV(D) compared to S. Typhimurium. (E) Expression of Sirt1 from cytoplasmic (C) and nuclear (N) fraction from BMDMs infected with delta ssrB. (F) Confocal image of Sirt1 and LysoTracker Red in delta ssrB-infected BMDMs. Sirt1-LysoTracker Red co-localization in untreated BMDMs infected with S. Typhimurium for 4h is shown for comparison (n = 3). (G) Immunoblot analysis of LC3 and p62. (H) Densitometric analysis of LC3 lipidation and p62 (n = 4). (I) Immunofluorescence image of delta ssrB-infected BMDMs stained for LC3 and LPS of S. Typhimurium (n = 3). (J) Quantitation of LC3 co-localization with SCVs. 100 SCVs were counted and expressed as percentage co-localization. (K) AKT, mTOR, p70S6K, NDRG1 expression upon S. Typhimurium (ST) and delta ssrB infection in BMDMs. (L) Densitometric analysis of AKT, mTOR, p70S6K and NDRG1 are shown from 3 independent experiments. Bar graphs are expressed as mean ± SEM, ***p≤0.001. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot S. Typhimurium mediated targeting of Sirt1 for lysosomal degradation is virulence dependent.Immunoblot analysis of ACC and LKB1 activation upon infection with delta ssrB(A) and delta ssaV(B) compared to S. Typhimurium. Sirt1 and acetylated-NF kappa B from macrophages infected with delta ssrB(C)and delta ssaV(D) compared to S. Typhimurium. (E) Expression of Sirt1 from cytoplasmic (C) and nuclear (N) fraction from BMDMs infected with delta ssrB. (F) Confocal image of Sirt1 and LysoTracker Red in delta ssrB-infected BMDMs. Sirt1-LysoTracker Red co-localization in untreated BMDMs infected with S. Typhimurium for 4h is shown for comparison (n = 3). (G) Immunoblot analysis of LC3 and p62. (H) Densitometric analysis of LC3 lipidation and p62 (n = 4). (I) Immunofluorescence image of delta ssrB-infected BMDMs stained for LC3 and LPS of S. Typhimurium (n = 3). (J) Quantitation of LC3 co-localization with SCVs. 100 SCVs were counted and expressed as percentage co-localization. (K) AKT, mTOR, p70S6K, NDRG1 expression upon S. Typhimurium (ST) and delta ssrB infection in BMDMs. (L) Densitometric analysis of AKT, mTOR, p70S6K and NDRG1 are shown from 3 independent experiments. Bar graphs are expressed as mean ± SEM, ***p≤0.001. Scale bar = 10μm for microscopical images. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28192515), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GAPDH
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36‑40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151‑155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over amino acid 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.
Product Datasheets
Citations for Human/Mouse/Rat GAPDH Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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AZD6738 Inhibits fibrotic response of conjunctival fibroblasts by regulating checkpoint kinase 1/P53 and PI3K/AKT pathways
Authors: Longxiang Huang, Qin Ye, Chunlin Lan, Xiaohui Wang, Yihua Zhu
Frontiers in Pharmacology
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Overlooked benefits of using polyclonal antibodies
Authors: Carl A Ascoli, Birte Aggeler
BioTechniques
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A20 protein regulates lipopolysaccharide-induced acute lung injury by downregulation of NF-?B and macrophage polarization in rats
Authors: Y Wang, Z Song, J Bi, J Liu, L Tong, Y Song, C Bai, X Zhu
Mol Med Rep, 2017-08-07;0(0):.
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Short-term exposure to dexamethasone promotes autonomic imbalance to the heart before hypertension
Authors: Francine Duchatsch, Paula B. Constantino, Naiara A. Herrera, Mayara F. Fabrício, Lidieli P. Tardelli, Aline M. Martuscelli et al.
Journal of the American Society of Hypertension
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Different activity patterns control various stages of Reelin synthesis in the developing neocortex
Authors: Kira Engeroff, Davide Warm, Stefan Bittner, Oriane Blanquie
Cerebral Cortex
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Glomerulonephritis and autoimmune vasculitis are independent of P2RX7 but may depend on alternative inflammasome pathways
Authors: Maria Prendecki, Stephen P McAdoo, Tabitha Turner‐Stokes, Ana Garcia‐Diaz, Isabel Orriss, Kevin J Woollard et al.
The Journal of Pathology
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Enhancing viability and angiogenic efficacy of mesenchymal stem cells via HSP90 alpha and HSP27 regulation based on ROS stimulation for wound healing
Authors: Inwoo Seo, Sung‐Won Kim, Jiyu Hyun, Yu‐Jin Kim, Hyun Su Park, Jeong‐Kee Yoon et al.
Bioengineering & Translational Medicine
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Pharmacological Activation of Non-canonical NF-kappa B Signaling Activates Latent HIV-1 Reservoirs In Vivo
Authors: Lars Pache, Matthew D. Marsden, Peter Teriete, Alex J. Portillo, Dominik Heimann, Jocelyn T. Kim et al.
Cell Reports Medicine
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Recombinant Lactaptin Induces Immunogenic Cell Death and Creates an Antitumor Vaccination Effect in Vivo with Enhancement by an IDO Inhibitor
Authors: Olga Troitskaya, Mikhail Varlamov, Anna Nushtaeva, Vladimir Richter, Olga Koval
Molecules
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Disruption of miR-29 Leads to Aberrant Differentiation of Smooth Muscle Cells Selectively Associated with Distal Lung Vasculature
Authors: Leah Cushing, Stefan Costinean, Wei Xu, Zhihua Jiang, Lindsey Madden, Pingping Kuang et al.
PLOS Genetics
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Role of p53 in Cisplatin-Induced Myotube Atrophy
Authors: Matsumoto C, Sekine H, Zhang N et al.
International Journal of Molecular Sciences
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miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4
Authors: Yujing Fan, Kuanyu Wang
Molecular Medicine Reports
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USP17 is required for peripheral trafficking of lysosomes
Authors: Jia Lin, Aidan P McCann, Naphannop Sereesongsaeng, Jonathan M Burden, Alhareth A Alsa’d, Roberta E Burden et al.
EMBO reports
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miR-34a Inhibits Proliferation and Invasion of Bladder Cancer Cells by Targeting Orphan Nuclear Receptor HNF4G
Authors: Huaibin Sun, Jun Tian, Wanhua Xian, Tingting Xie, Xiangdong Yang
Disease Markers
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A methyl-deficient diet fed to rats during the pre- and peri-conception periods of development modifies the hepatic proteome in the adult offspring
Authors: Christopher A. Maloney, Susan M. Hay, Martin D. Reid, Gary Duncan, Fergus Nicol, Kevin D. Sinclair et al.
Genes & Nutrition
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Discovery and Characterization of ZL-2201, a Potent, Highly Selective, and Orally Bioavailable Small-molecule DNA-PK Inhibitor.
Authors: Lal S, Bhola N H et al.
Cancer Res Commun
Species: Human
Sample Types:
Applications: Simple Western -
Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC
Authors: Benavente, MCR;Hakeem, ZA;Davis, AR;Murray, NB;Azadi, P;Mace, EM;Barb, AW;
Scientific reports
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Regulation of angiogenesis by endocytic trafficking mediated by cytoplasmic dynein 1 light intermediate chain 1
Authors: Johnson, D;Colijn, S;Richee, J;Yano, J;Burns, M;Davis, AE;Pham, VN;Saric, A;Jain, A;Yin, Y;Castranova, D;Melani, M;Fujita, M;Grainger, S;Bonifacino, JS;Weinstein, BM;Stratman, AN;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
An IBD-associated pathobiont synergises with NSAID to promote colitis which is blocked by NLRP3 inflammasome and Caspase-8 inhibitors
Authors: Singh R, Rossini V, Stockdale SR et al.
Gut Microbes
-
Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice
Authors: CJ Chen, CC Wu, CY Chang, JR Li, YC Ou, WY Chen, SL Liao, JD Wang
International Journal of Molecular Sciences, 2022-08-15;23(16):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Western Blot -
Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis
Authors: W Xiao, A Pinilla-Ba, J Faulkner, X Song, P Prabhakar, H Qiu, KW Moremen, A Ludwig, PJ Dempsey, P Azadi, L Wang
Scientific Reports, 2022-03-14;12(1):4352.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro
Authors: Y Basmaeil, E Bahattab, A Al Subayyi, HB Kulayb, M Alrodayyan, M Abumaree, T Khatlani
Cells, 2021-12-10;10(12):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy
Authors: TA Shah, HK Pallera, CL Kaszowski, WT Bass, FA Lattanzio
Frontiers in Neuroscience, 2021-02-12;15(0):616734.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB
Authors: T Guerenne-D, V Couderc, L Duponchel, V Sol, P Leproux, JM Petit
Sci Rep, 2020-10-07;10(1):16749.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
Cancer Cell Int, 2020-10-02;20(0):476.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mouse WIF1 Is Only Modified with O-Fucose in Its EGF-like Domain III Despite Two Evolutionarily Conserved Consensus Sites
Authors: F Pennarubia, E Pinault, B Al Jaam, CE Brun, A Maftah, A Germot, S Legardinie
Biomolecules, 2020-08-28;10(9):.
Species: Hamster
Sample Types: Cell Culture Supernates
Applications: Western Blot -
scAAV2-Mediated C3 Transferase Gene Therapy in a Rat Model with Retinal Ischemia/Reperfusion Injuries
Authors: J Tan, X Zhang, D Li, G Liu, Y Wang, D Zhang, X Wang, W Tian, X Dong, L Zhou, X Zhu, X Liu, N Fan
Mol Ther Methods Clin Dev, 2020-04-25;17(0):894-903.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
C3 Transferase-Expressing scAAV2 Transduces Ocular Anterior Segment Tissues and Lowers Intraocular Pressure in Mouse and Monkey
Authors: J Tan, X Wang, S Cai, F He, D Zhang, D Li, X Zhu, L Zhou, N Fan, X Liu
Mol Ther Methods Clin Dev, 2019-11-29;17(0):143-155.
Species: Human
Sample Types: Cell
Applications: Western Blot -
PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo
Authors: L Liu, J Cao, J Zhao, X Li, Z Suo, H Li
Onco Targets Ther, 2019-11-18;12(0):9899-9913.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway
Authors: A Der Vartan, J Chabanais, C Carrion, A Maftah, A Germot
Int J Mol Sci, 2019-09-06;20(18):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms
Authors: MS Abdelfatta, MIY Elmallah, HY Ebrahim, RS Almeer, RMA Eltanany, AE Abdel Mone
PLoS ONE, 2019-06-13;14(6):e0216737.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism
Authors: T Liu, NA Barrett, Y Kanaoka, K Buchheit, TM Laidlaw, D Garofalo, J Lai, HR Katz, C Feng, JA Boyce
Mucosal Immunol, 2019-01-21;0(0):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium
Authors: B Ilyas, DT Mulder, DJ Little, W Elhenawy, MM Banda, D Pérez-Mora, CN Tsai, NYE Chau, VH Bustamante, BK Coombes
Cell Rep, 2018-10-23;25(4):825-832.e5.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
Cell. Physiol. Biochem., 2017-12-08;44(5):1896-1911.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis
Authors: MK Shim, HY Yoon, S Lee, MK Jo, J Park, JH Kim, SY Jeong, IC Kwon, K Kim
Sci Rep, 2017-11-30;7(1):16635.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
Sci Rep, 2017-07-11;7(1):5081.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy
Authors: R Ganesan, NJ Hos, S Gutierrez, J Fischer, JM Stepek, E Daglidu, M Krönke, N Robinson
PLoS Pathog, 2017-02-13;13(2):e1006227.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells
Authors: CT Yang, R Ma, RA Axton, M Jackson, AH Taylor, A Fidanza, L Marenah, J Frayne, JC Mountford, LM Forrester
Stem Cells, 2017-01-19;35(4):886-897.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
Invest Ophthalmol Vis Sci, 2014-05-27;55(7):4276-87.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice.
Authors: Chen, Felicia, Marquez, Hector, Kim, Youn-Kyu, Qian, Jun, Shao, Fengzhi, Fine, Alan, Cruikshank, William, Quadro, Loredana, Cardoso, Wellingt
J Clin Invest, 2014-01-09;124(2):801-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function.
Authors: Zhi, Hui, Luptak, Ivan, Alreja, Gaurav, Shi, Jianru, Guan, Jian, Metes-Kosik, Nicole, Joseph, Jacob
PLoS ONE, 2013-12-11;8(12):e81612.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
BMC Cancer, 2012-05-29;12(0):201.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Overexpression of carbonic anhydrase and HIF-1alpha in Wilms tumours.
Authors: Dungwa JV, Hunt LP, Ramani P
BMC Cancer, 2011-09-12;11(0):390.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
MicroRNA?181 exerts an inhibitory role during renal fibrosis by targeting early growth response factor?1 and attenuating the expression of profibrotic markers
Authors: X Zhang, Z Yang, Y Heng, C Miao
Mol Med Rep, 2019-02-18;0(0):.
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Peptidic inhibitors of insulin-degrading enzyme with potential for dermatological applications discovered via phage display
Authors: Caitlin N. Suire, Sarah Nainar, Michael Fazio, Adam G. Kreutzer, Tara Paymozd-Yazdi, Caitlyn L. Topper et al.
PLOS ONE
-
Quantitating drug-target engagement in single cells in vitro and in vivo
Authors: J Matthew Dubach, Eunha Kim, Katherine Yang, Michael Cuccarese, Randy J Giedt, Labros G Meimetis et al.
Nature Chemical Biology
-
Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer
Authors: Naphannop Sereesongsaeng, Sara H. McDowell, James F. Burrows, Christopher J. Scott, Roberta E. Burden
Breast Cancer Research
-
An IBD-associated pathobiont synergises with NSAID to promote colitis which is blocked by NLRP3 inflammasome and Caspase-8 inhibitors
Authors: Singh R, Rossini V, Stockdale SR et al.
Gut Microbes
-
Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing
Authors: Sang Ho Lee, Yu-Jin Kim, Yeong Hwan Kim, Han Young Kim, Suk Ho Bhang
Stem Cell Research & Therapy
-
Spinal interleukin-6 contributes to central sensitisation and persistent pain hypersensitivity in a model of juvenile idiopathic arthritis
Authors: Charlie H.T. Kwok, Annastazia E Learoyd, Julia Canet-Pons, Tuan Trang, Maria Fitzgerald
Brain, Behavior, and Immunity
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Synthesis and biological evaluation of 3-(1,3,4-oxadiazol-2-yl)-1,8-naphthyridin-4(1H)-ones as cisplatin sensitizers
Authors: Xueyan Hou, Hao Luo, Mengqi Zhang, Guoyi Yan, Chunlan Pu, Suke Lan et al.
MedChemComm
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Modulation of proteoglycan receptor PTP? enhances MMP-2 activity to promote recovery from multiple sclerosis
Authors: F Luo, AP Tran, L Xin, C Sanapala, BT Lang, J Silver, Y Yang
Nat Commun, 2018-10-08;9(1):4126.
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POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
Authors: Julien Chabanais, François Labrousse, Alain Chaunavel, Agnès Germot, Abderrahman Maftah
Cancers (Basel)
-
Mechanism of intrinsic resistance of lung squamous cell carcinoma to epithelial growth factor receptor‑tyrosine kinase inhibitors revealed by high‑throughput RNA interference screening
Authors: Lixia Ju, Zhiyi Dong, Juan Yang, Minghua Li
Oncology Letters
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Area light source‐triggered latent angiogenic molecular mechanisms intensify therapeutic efficacy of adult stem cells
Authors: Yu‐Jin Kim, Sung‐Won Kim, Gwang‐Bum Im, Yeong Hwan Kim, Gun‐Jae Jeong, Hye Ran Jeon et al.
Bioengineering & Translational Medicine
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Constitutive LH receptor activity impairs NO-mediated penile smooth muscle relaxation
Authors: Deepak S. Hiremath, Fernanda B.M. Priviero, R. Clinton Webb, CheMyong Ko, Prema Narayan
Reproduction
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The blot contains HeLa cell lysate (1, 2, 3 and 5 µg) spiked with 200 ng of human transferrin per lane. Transferrin is detected in the red channel, tubulin is detected in the green channel and GAPDH is detected in the blue channel.
