Home / Glutathione Peroxidase 1/GPX1 / Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antibody

Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antibody

Catalog #: AF3798
30 Citations Datasheet / COA / SDS
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AF3798
AF3798-SP
Glutathione Peroxidase 1/GPX1 in Rat Liver.
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Citations (30)
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Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat GPX1. In Western blots, this antibody shows no cross-reactivity with recombinant human GPX2 or GPX3.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Glutathione Peroxidase 1
Gly48-Ala201
Accession # P07203
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
Glutathione Peroxidase 1/GPX1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Glutathione Peroxidase 1/GPX1 knockoutHEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Glutathione Peroxidase 1/GPX1 antibody in Rat Liver by Immunohistochemistry (IHC-Fr). View Larger

Glutathione Peroxidase 1/GPX1 in Rat Liver. Glutathione Peroxidase 1/GPX1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Human/Mouse/Rat Glutathione Peroxidase 1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Glutathione Peroxidase 1 by Western Blot. Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human and Mouse Glutathione Peroxidase 1/GPX1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse Glutathione Peroxidase 1/GPX1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human Glutathione Peroxidase 1/GPX1 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Glutathione Peroxidase 1/GPX1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Glutathione Peroxidase 1/GPX1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 24 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse Glutathione Peroxidase 1/GPX1 by Western Blot View Larger

Detection of Mouse Glutathione Peroxidase 1/GPX1 by Western Blot Induction of oxidative stress defense during muscle mild mitochondrial stress. (A) Skeletal muscle mRNA expression in quadriceps muscle of oxidative stress defense markers of 24 wks old animals (n = 8–10). (B and C) Representative immunoblots of glutathione peroxidase 1 (GPX1) and GPX4 from of cropped gels using alpha Tubulin as loading control (2 samples shown out of 4–5 analyzed per group). (D) Quantification of GPX1 and GPX4 protein expression in quadriceps muscle from 24 wks old mice (4–5 per group). (E) Skeletal muscle catalase activity of 24 wks old animals (n = 5–10). (F) Skeletal muscle total GPX activity of 24 wks old animals (n = 7–10). (G) Representative immunoblots of methylglyoxal advanced glycation end-products (AGEs) in quadriceps muscle from 24 wks old mice using alpha Tubulin ( alpha TUB) as loading control (2 samples shown out of 4–5 analyzed per group). All absolute values are expressed as box-and-whisker plots, relative or normalized data are expressed as means + SEM; *p < 0.05; **p < 0.01; ***p < 0.001. (H) Graphical summary of Nrf2-dependent and Nrf2-independent adaptive cell-autonomous stress response pathways during respiratory uncoupling-induced muscle (mild) mitochondrial stress. This cartoon was created using Servier Medical Art (https://smart.servier.com). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-20901-4), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione Peroxidase 1/GPX1

Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201 amino acid, 23 kDa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX1 is a 92 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX1 is an important antioxidant enzyme and protects red blood cells and other cells from oxidative damage. Deficiencies in red cell GPX1 have been associated with hemolytic anemia. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Entrez Gene IDs
2876 (Human); 14775 (Mouse); 24404 (Rat)
Alternate Names
Cellular glutathione peroxidase; CGPx; EC 1.11.1; EC 1.11.1.9; Glutathione Peroxidase 1; GPX1; GPx-1; GSHPX1; GSHPx-1; MGC14399; MGC88245

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Citations for Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. N-Acetylcysteine Reduces the Pro-Oxidant and Inflammatory Responses during Pancreatitis and Pancreas Tumorigenesis
    Authors: Minati MA, Libert M, Dahou H et al.
    Antioxidants (Basel)
  2. Prolonged maternal exposure to glucocorticoids alters selenoprotein expression in the developing brain
    Authors: Pamela Toh, Lucia A. Seale, Marla J. Berry, Daniel J. Torres
    Frontiers in Molecular Neuroscience
  3. Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen
    Authors: Sara Evke, Qishan Lin, Juan Andres Melendez, Thomas John Begley
    Genes (Basel)
  4. N-Acetylcysteine Reduces the Pro-Oxidant and Inflammatory Responses during Pancreatitis and Pancreas Tumorigenesis
    Authors: Minati MA, Libert M, Dahou H et al.
    Antioxidants (Basel)
  5. Juvenile Selenium Deficiency Impairs Cognition, Sensorimotor Gating, and Energy Homeostasis in Mice
    Authors: Victor W. Kilonzo, Alexandru R. Sasuclark, Daniel J. Torres, Celine Coyle, Jennifer M. Pilat, Christopher S. Williams et al.
    Frontiers in Nutrition
  6. Huanglian‑Houpo drug combination ameliorates H1N1‑induced mouse pneumonia via cytokines, antioxidant factors and TLR/MyD88/NF‑ kappa B signaling pathways
    Authors: Li Zhang, Bei Zhang, Linjing Wang, Mingwu Lou, Yunxia Shen
    Experimental and Therapeutic Medicine
  7. Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species
    Authors: Vuong Cat Khanh, Toshiharu Yamashita, Kinuko Ohneda, Chiho Tokunaga, Hideyuki Kato, Motoo Osaka et al.
    Scientific Reports
  8. The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene
    Authors: Andrea Leonardi, Nataliia Kovalchuk, Lei Yin, Lauren Endres, Sara Evke, Steven Nevins et al.
    Epigenetics
  9. Glutathione peroxidase-1 overexpression reduces oxidative stress, and improves pathology and proteome remodeling in the kidneys of old mice
    Authors: Y Chu, RS Lan, R Huang, H Feng, R Kumar, S Dayal, KS Chan, DF Dai
    Aging Cell, 2020-05-13;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Loss of epitranscriptomic control of selenocysteine utilization engages senescence and mitochondrial reprogramming?
    Authors: MY Lee, A Leonardi, TJ Begley, JA Melendez
    Redox Biol, 2019-11-11;28(0):101375.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. A Mitochondrial Specific Antioxidant Reverses Metabolic Dysfunction and Fatty Liver Induced by Maternal Cigarette Smoke in Mice
    Authors: G Li, YL Chan, S Sukjamnong, AG Anwer, H Vindin, M Padula, R Zakarya, J George, BG Oliver, S Saad, H Chen
    Nutrients, 2019-07-21;11(7):.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  12. Reversal of right ventricular failure by chronic alpha 1A-subtype adrenergic agonist therapy
    Authors: Patrick M. Cowley, Guanying Wang, Philip M. Swigart, Anaha Raghunathan, Nikitha Reddy, Pranavi Dulam et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  13. Phosphate Binder, Ferric Citrate, Attenuates Anemia, Renal Dysfunction, Oxidative Stress, Inflammation, and Fibrosis in 5/6 Nephrectomized CKD Rats
    Authors: Wanghui Jing, Ane C. F. Nunes, Ted Farzaneh, Mahyar Khazaeli, Wei Ling Lau, Nosratola D. Vaziri
    Journal of Pharmacology and Experimental Therapeutics
  14. Evidence supporting oxidative stress in a moderately affected area of the brain in Alzheimer's disease
    Authors: P Youssef, B Chami, J Lim, T Middleton, GT Sutherland, PK Witting
    Sci Rep, 2018-08-01;8(1):11553.
    Species: Human
    Sample Types: Tissue Homogenates
  15. Dietary tetrahydrocurcumin reduces renal fibrosis and cardiac hypertrophy in 5/6 nephrectomized rats
    Authors: WL Lau, M Khazaeli, J Savoj, K Manekia, M Bangash, RG Thakurta, A Dang, ND Vaziri, B Singh
    Pharmacol Res Perspect, 2018-02-19;6(2):e00385.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Partial involvement of Nrf2 in skeletal muscle mitohormesis as an adaptive response to mitochondrial uncoupling
    Authors: V Coleman, P Sa-Nguanmo, J Koenig, TJ Schulz, T Grune, S Klaus, AP Kipp, M Ost
    Sci Rep, 2018-02-05;8(1):2446.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Sexual Dimorphism in the Selenocysteine Lyase Knockout Mouse
    Authors: AN Ogawa-Wong, AC Hashimoto, H Ha, MW Pitts, LA Seale, MJ Berry
    Nutrients, 2018-01-31;10(2):.
    Species: Mouse
    Sample Types: Cell Lysate
    Applications: Western Blot
  18. LCZ696 (Sacubitril/valsartan) ameliorates oxidative stress, inflammation, fibrosis and improves renal function beyond angiotensin receptor blockade in CKD
    Authors: W Jing, ND Vaziri, A Nunes, Y Suematsu, T Farzaneh, M Khazaeli, H Moradi
    Am J Transl Res, 2017-12-15;9(12):5473-5484.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. eIF2 alpha phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet
    Authors: Woo-Gyun Choi, Jaeseok Han, Ji-Hyeon Kim, Mi-Jeong Kim, Jae-Woo Park, Benbo Song et al.
    Nutrition & Metabolism
  20. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis
    Nat Chem Biol, 2016-05-09;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Sigma 1 receptor regulates the oxidative stress response in primary retinal Müller glial cells via NRF2 signaling and system xc(-), the Na(+)-independent glutamate-cystine exchanger
    Authors: Jing Wang, Arul Shanmugam, Shanu Markand, Eric Zorrilla, Vadivel Ganapathy, Sylvia B. Smith
    Free Radical Biology and Medicine
  22. Oxidative stress and altered lipid homeostasis in the programming of offspring fatty liver by maternal obesity
    Authors: Maria Z. Alfaradhi, Denise S. Fernandez-Twinn, Malgorzata S. Martin-Gronert, Barbara Musial, Abigail Fowden, Susan E. Ozanne
    American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
  23. Roles of Glutathione in Antioxidant Defense, Inflammation, and Neuron Differentiation in the Thalamus of HIV-1 Transgenic Rats
    Authors: Xiaosha Pang, Jun Panee
    Journal of Neuroimmune Pharmacology
  24. Isoform-specific binding of selenoprotein P to the beta-propeller domain of apolipoprotein E receptor 2 mediates selenium supply.
    Authors: Kurokawa S, Bellinger F, Hill K, Burk R, Berry M
    J Biol Chem, 2014-02-13;289(13):9195-207.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Mice lacking selenoprotein P and selenocysteine lyase exhibit severe neurological dysfunction, neurodegeneration, and audiogenic seizures.
    Authors: Byrns, China N, Pitts, Matthew, Gilman, Christy, Hashimoto, Ann C, Berry, Marla J
    J Biol Chem, 2014-02-11;289(14):9662-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  26. Evaluation of selenite effects on selenoproteins and cytokinome in human hepatoma cell lines.
    Authors: Rusolo, Fabiola, Pucci, Biagio, Colonna, Giovanni, Capone, Francesc, Guerriero, Eliana, Milone, Maria Ri, Nazzaro, Melissa, Volpe, Maria Gr, Di Bernardo, Gianni, Castello, Giuseppe, Costantini, Susan
    Molecules, 2013-02-26;18(3):2549-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Prostaglandin H Synthase-2-catalyzed Oxygenation of 2-Arachidonoylglycerol Is More Sensitive to Peroxide Tone than Oxygenation of Arachidonic Acid.
    Authors: Musee J, Marnett L
    J Biol Chem, 2012-09-01;287(44):37383-94.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Disruption of the selenocysteine lyase-mediated selenium recycling pathway leads to metabolic syndrome in mice.
    Authors: Seale L, Hashimoto A, Kurokawa S, Gilman C, Seyedali A, Bellinger F, Raman A, Berry M
    Mol Cell Biol, 2012-08-13;32(20):4141-54.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  29. Resveratrol reduces endothelial oxidative stress by modulating the gene expression of superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1) and NADPH oxidase subunit (Nox4).
    Authors: Spanier G, Xu H, Xia N
    J. Physiol. Pharmacol., 2009-10-01;60(0):111-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease.
    Authors: Pitts MW, Kremer PM, Hashimoto AC et al.
    J. Neurosci.

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