Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: GRIN2A/NMDAR2A

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg⁺⁺ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitute the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.

Product Datasheets

Related Research Areas

• Glutamate Receptors
• Neurodegenerative Disease
• Neurotransmitter Receptors, Transporters, and Ion Channels
• The Glutamatergic System in Alzheimer's Disease