Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

Catalog #: PPS055 Datasheet / COA / SDS

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PPS055

Detection of GRIN2B/NMDAR2B by Western Blot.

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Human/Mouse/Rat GRIN2B/NMDAR2B Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Human, mouse, and rat ~180 kDa NMDA NR2B

Source

Polyclonal Rabbit Serum

Purification

Antigen Affinity-purified

Immunogen

NMDA R, NR2B Subunit

Formulation

50 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GRIN2B/NMDAR2B by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDA Receptor.

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Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, -20 °C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg⁺⁺ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2B form generates a high conductance NMDAR. Human NR2B (also NR3 and GRIN2B) is a 180 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD) and a 646 aa cytoplasmic region. The loop connecting TM segments 2 and 3, plus the N-terminal ECD constitute the glutamate binding site. Human NR2B is more than 98% aa identical to NR2B in mouse, rat, and dog.

References

Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

Entrez Gene IDs

2904 (Human)

Alternate Names

DEE101;GluN1;Glutamate receptor;Glutamate receptor ionotropic, NMDA 1;GRIN1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;NR1; GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B