Human/Mouse/Rat GRIN2C/NMDAR2C Antibody

Catalog #: PPS033
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PPS033

Detection of GRIN2C/NMDAR2C by Western Blot.

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Human/Mouse/Rat GRIN2C/NMDAR2C Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Human, mouse, rat ~140 kDa NMDAR NR2C subunit

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Fusion protein from the NR2C subunit of the NMDA Receptor

Formulation

10 μg per vial; lyophilized in 5 mM ammonium bicarbonate.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Immunoprecipitation

3 μL /200 μg lysate

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GRIN2C/NMDAR2C by Western Blot. Western blot of 10 μg of rat brain (cerebellum) lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-Terminal NR2C subunit NMDA Receptor fusion protein used as the antigen.

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Preparation and Storage

Reconstitution

This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂PO₄, pH 7.4) before use.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

The lyophilized product is stable at ≤ -20° C for at least 1 year. After reconstitution the antibody should be aliquoted and stored at ≤ -20° C.

Background: GRIN2C/NMDAR2C

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650 - 850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg⁺⁺ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2C form generates a low conductance NMDAR. This receptor is particularly abundant in the cerebellum and thalamus. Rat NR2C is a 180 kDa, 1237 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 535 aa extracellular domain (ECD) and a 401 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD likely constitute the glutamate binding site. Rat NR2C shares 97% and 88% aa sequence identity to mouse and human NR2C, respectively.

References

Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16.
Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
Monyer, H. et al. (1992) Science 256:1217.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2C

Entrez Gene IDs

2905 (Human); 14813 (Mouse); 24411 (Rat)

Alternate Names

glutamate [NMDA] receptor subunit epsilon-3; glutamate receptor, ionotropic, N-methyl D-aspartate 2C; GRIN2C; NMDA R, NR2C Subunit; NMDAR2C; NMDAR2CNR2C; N-methyl D-aspartate receptor subtype 2C; N-methyl-D-aspartate receptor subunit 2C; NR2C

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Citation for Human/Mouse/Rat GRIN2C/NMDAR2C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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