Human/Mouse/Rat GRK2 Antibody

Catalog #: MAB43391 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB43391-100
MAB43391-SP

Detection of Human, Mouse, and Rat GRK2 by Western Blot.

3 Images

All GRK2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat GRK2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human GRK2 in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2089B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human GRK2
Pro468-Leu689
Accession # P25098

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

1 µg/mL

See below

Knockout Validated

GRK2 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in GRK2 knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat GRK2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line, LL/2 mouse Lewis lung carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human GRK2 by Simple Western^TM. Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK2 at approximately 80 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/ Rat GRK2 Monoclonal Antibody (Catalog # MAB43391). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated

Western Blot Shows Human GRK2 Antibody Specificity by Using Knockout Cell Line.

View Larger

Western Blot Shows Human GRK2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and GRK2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK2

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the beta ARK subfamily, and was originally termed beta ‑adrenergic receptor kinase 1 ( beta ARK1).

Long Name

G protein-coupled Receptor Kinase 2

Entrez Gene IDs

156 (Human); 110355 (Mouse); 25238 (Rat)

Alternate Names

ADRBK1; adrenergic, beta, receptor kinase 1; BARK; BARK1; BARK1beta-ARK-1; Beta-ARK1; Beta-ARK-1; EC 2.7.11; EC 2.7.11.15; FLJ16718; G-protein coupled receptor kinase 2; GRK2; GRK2beta-adrenergic receptor kinase 1