Home / Hepassocin/FGL1 / Human/Mouse/Rat Hepassocin/FGL1 Antibody

Human/Mouse/Rat Hepassocin/FGL1 Antibody

Catalog #: AF1614
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1614
AF1614-SP
Detection of Human, Mouse, and Rat Hepassocin/FGL1 by Western Blot.
4 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Hepassocin/FGL1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Hepassocin/FGL1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
Hep3B human hepatocellular carcinoma cell line, human liver
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Hepassocin/FGL1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Hepassocin/FGL1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of Hepassocin/FGL1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Hepassocin/FGL1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF1614, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules.

Simple Western View Larger

Detection of Human Hepassocin/FGL1 by Simple WesternTM. Simple Western lane view shows lysates of Hep3B human hepatocellular carcinoma cell line, human liver, loaded at 0.2 mg/mL. A specific band was detected for Hepassocin/FGL1 at approximately 41 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot View Larger

Detection of Human Hepassocin/FGL1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein (HFREP-1), is a liver specific secreted protein containing one fibrinogen C-terminal domain. It exists as a homodimer and is reported to have hepatocyte mitogenic activity (1).

References
  1. 1.Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
Long Name
Fibrinogen Like 1
Entrez Gene IDs
2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)
Alternate Names
FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human/Mouse/Rat Hepassocin/FGL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot.
    Authors: Rijken DC, Dirkx SP, Luider TM, Leebeek FW
    Biochem. Biophys. Res. Commun., 2006-09-15;350(1):191-4.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Mass Spectrometry, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Hepassocin/FGL1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Hepassocin/FGL1 Antibody and earn rewards!

Have you used Human/Mouse/Rat Hepassocin/FGL1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review