Human/Mouse/Rat Histone H2AX Antibody Summary
Gly3-Ser131
Accession # P16104
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Histone H2AX by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AX Monoclonal Antibody (Catalog # MAB3406) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Histone H2AX at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Histone H2AX in HeLa Human Cell Line. Histone H2AX was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Histone H2AX Monoclonal Antibody (Catalog # MAB3406) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone H2AX
Histone H2AX is a core histone protein that is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.
Product Datasheets
Citations for Human/Mouse/Rat Histone H2AX Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Inflammasome Coordinates Senescent Chronic Wound Induced by Thalassophryne nattereri Venom
Authors: Lima, C;Andrade-Barros, AI;Carvalho, FF;Falc�o, MAP;Lopes-Ferreira, M;
International journal of molecular sciences
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
NAMPT-dependent NAD+ salvage is crucial for the decision between apoptotic and necrotic cell death under oxidative stress
Authors: Takuto Nishida, Isao Naguro, Hidenori Ichijo
Cell Death Discovery
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Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines
Authors: A Schepsky, G Asta Traus, J Petur Joel, S Ingthorsso, J Kricker, J Thor Bergt, A Asbjarnars, T Gudjonsson, N Nupponen, A Slipicevic, F Lehmann, T Gudjonsson
Cancer Med, 2020-07-27;0(0):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Quantitative analysis of ATM phosphorylation in lymphocytes
Authors: CJ Bakkenist, RK Czambel, Y Lin, NA Yates, X Zeng, J Shogan, JC Schmitz
DNA Repair (Amst.), 2019-06-04;80(0):1-7.
Species: Human
Sample Types: Whole Cells
Applications: IR Fluorescence -
Phenotypic Plasticity of Invasive Edge Glioma Stem-like Cells in Response to Ionizing Radiation
Authors: M Minata, A Audia, J Shi, S Lu, J Bernstock, MS Pavlyukov, A Das, SH Kim, YJ Shin, Y Lee, H Koo, K Snigdha, I Waghmare, X Guo, A Mohyeldin, D Gallego-Pe, J Wang, D Chen, P Cheng, F Mukheef, M Contreras, JF Reyes, B Vaillant, EP Sulman, SY Cheng, JM Markert, BA Tannous, X Lu, M Kango-Sing, LJ Lee, DH Nam, I Nakano, KP Bhat
Cell Rep, 2019-02-12;26(7):1893-1905.e7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice
Authors: S Wichaiyo, S Lax, SJ Montague, Z Li, B Grygielska, JA Pike, EJ Haining, A Brill, SP Watson, J Rayes
Haematologica, 2019-02-07;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
miR-24-mediated knockdown of H2AX damages mitochondria and the insulin signaling pathway
Authors: JH Jeong, Y Cheol Kang, Y Piao, S Kang, YK Pak
Exp. Mol. Med., 2017-04-07;49(4):e313.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neutrophil extracellular traps induce IL-1 beta production by macrophages in combination with lipopolysaccharide
Authors: Zhongshuang Hu, Taisuke Murakami, Hiroshi Tamura, Johannes Reich, Kyoko Kuwahara-Arai, Toshiaki Iba et al.
International Journal of Molecular Medicine
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The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
Oncogene, 2014-09-29;34(29):3826-38.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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10 micrograms of mouse cortex
antibody used at 1/1300
1 second exposition
a: membrane after blotting with 15kDa molecular weight (*)
b : membrane after blotting with 20kDa molecular weight (**)
