Human/Mouse/Rat Histone H2AY Antibody Summary
Gly230-Thr348
Accession # O75367
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Histone H2AY by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone H2AY at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Histone H2AY in HeLa Human Cell Line. Histone H2AY was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone H2AY
Histone H2AY, also known as macro H2A1 (MH2A1) and H2AFY, is a 40 kDa variant of the histone family, basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Ubiquitously expressed, H2AY replaces conventional H2A histones in a subset of nucleosomes, where it represses transcription and participates in stable X chromosome inactivation. H2AY expression is also enriched in senescence-associated heterochromatin. Alternative splicing creates multiple transcript variants, encoding three distinct isoforms. H2AY expressed in blood has been identified as a potential biomarker associated with the activity and therapeutic response of Huntington disease, an autosomal dominant neurodegenerative disorder. Over amino acids 230-348, human H2AY shares 98% identity with mouse and rat H2AY.
Product Datasheets
Citations for Human/Mouse/Rat Histone H2AY Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration
Authors: Guo Y, Chomiak AA, Hong Y et al.
iScience
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Nde1 is required for heterochromatin compaction and stability in neocortical neurons
Authors: Chomiak AA, Guo Y, Kopsidas CA et al.
iScience
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Nde1 is required for heterochromatin compaction and stability in neocortical neurons
Authors: Chomiak AA, Guo Y, Kopsidas CA et al.
iScience
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