Human/Mouse/Rat HMGB1/HMG-1 Antibody Summary
Accession # P09429
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat HMGB1/HMG‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Hepa 1-6 mouse hepatoma cell line, H4-II-E-C3 rat hepatoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB16901) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for HMGB1/HMG-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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HMGB1/HMG‑1 in Human Prostate Cancer Tissue. HMGB1/HMG-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rat Anti-Human/Mouse/Rat HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB16901) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human and Mouse HMGB1/HMG‑1 by Simple WesternTM. Simple Western shows lysates of Jurkat human acute T cell leukemia cell line and Hepa 1‑6 mouse hepatoma cell line, loaded at 0.2 mg/ml. A specific band was detected for HMGB1/HMG‑1 at approximately 35 kDa (as indicated) using 20 µg/mL of Rat Anti-Human/Mouse/Rat HMGB1/HMG‑1 Monoclonal Antibody (Catalog # MAB16901). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HMGB1/HMG-1
Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1‑3). Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu (4, 5). Residues 27‑43 and 178‑184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues (6). HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.
- Lotze, M.T. and K.J. Tracey (2005) Nat. Rev. Immunol. 5:331.
- Yang, H. et al. (2005) J. Leukoc. Biol. 78:1.
- Dumitriu, I.E. et al. (2005) Trends Immunol. 26:381.
- Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
- Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
- Bonaldi, T. et al. (2003) EMBO J. 22:5551.
- Muller, S. et al. (2001) EMBO J. 20:4337.
- Bustin, M. (1999) Mol. Cell. Biol. 19:5237.
- Wang, H. et al. (1999) Science. 285:248.
- Dimitriu, I.E. et al. (2005) J. Immunol. 174:7506.
- Najima, Y. et al. (2005) J. Biol. Chem. 280:27523.
- Gardella, S. et al. (2002) EMBO Rep. 3:995.
Product Datasheets
Citations for Human/Mouse/Rat HMGB1/HMG-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Gastroprotective effect of dapagliflozin in ethanol-induced gastric lesions in rats: Crosstalk between HMGB1/RAGE/PTX3 and TLR4/MyD88/VEGF/PDGF signaling pathways
Authors: RM Salama, RH Ahmed, AA Farid, BA AbdElSatta, RM AbdelBaset, ME Youssef, SA El Wakeel
International immunopharmacology, 2023-01-07;115(0):109686.
Species: Rat
Sample Types: Protein
Applications: Western Blot -
Protective Effect of an Anti-HMGB-1 Neutralizing Antibody on Hemozoin-Induced Alveolar Epithelial Cell in a Model of Malaria Associated ALI/ARDS
Authors: Tachpon Techarang, Pitchanee Jariyapong, Parnpen Viriyavejakul, Supattra Glaharn, Charit Srisook, Chuchard Punsawad
Iranian Journal of Parasitology
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Kakonein restores diabetes-induced endothelial junction dysfunction via promoting autophagy-mediated NLRP3 inflammasome degradation
Authors: D Lian, J Liu, R Han, J Jin, L Zhu, Y Zhang, Y Huang, X Wang, S Xian, Y Chen
Journal of Cellular and Molecular Medicine, 2021-06-27;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses
Authors: Xiaowen Bi, Baolin Jiang, Jinyi Zhou, Xirui Fan, Xintong Yan, Juanjuan Liang et al.
Frontiers in Immunology
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Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury and improved neurological deficits in rats
Authors: D Wang, K Liu, H Wake, K Teshigawar, S Mori, M Nishibori
Sci Rep, 2017-04-10;7(0):46243.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P
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