Human/Mouse/Rat HSP40 MAb (Clone 419401)

Name: Human/Mouse/Rat HSP40 MAb (Clone 419401)
Brand: R&D Systems
SKU: A-410
Price: 169 USD

Catalog #: A-410 Datasheet / COA / SDS

Discontinued Product

A-410 has been discontinued.

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Detection of Human HSP40/DNAJB1 by Western Blot.

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All HSP40/DNAJB1 Antibodies

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Human/Mouse/Rat HSP40 MAb (Clone 419401) Summary

Species Reactivity

Human, Mouse, Rat

Source

Monoclonal Mouse IgG2b Clone # 419401

Immunogen

E. coli-derived recombinant human HSP40/DNAJB1
Met1-Ile340

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

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Citations (1)

Detection of Lgr4/GPR48 antibody in HEK293 Human Cell Line Transfected with Human Lgr4/GPR48 and eGFP antibody by Flow Cytometry.

Human Lgr4/GPR48 PE-conjugated Antibody

Cat # FAB7750P

Applications

Recommended Concentration

Sample

Western Blot

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HSP40/DNAJB1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP40/DNAJB1 Monoclonal Antibody (Catalog # A-410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP40/DNAJB1 at approximately 37-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: HSP40/DNAJB1

Heat shock protein 40 kDa (HSP40) is the human homologue of the bacterial DnaJ heat shock protein. HSP40, also known as HSPF1 and DnaJ Homolog subfamily B member 1 (DNAJB1), is a 340 amino acid, 40 kDa member of the heat shock protein family. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP40 is a stress inducible chaperone that co-localizes with HSP70 and can bind unfolded proteins and prevent protein denaturation and aggregation. The conserved amino terminal J domain can interact with HSP70 and stimulate its ATPase activity.

Long Name

Heat Shock 40 kDa Protein

Entrez Gene IDs

3337 (Human); 81489 (Mouse); 361384 (Rat)

Alternate Names

DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ (Hsp40) homolog, subfmaily B, member 1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; DNAJB1; Hdj1; hDj-1; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; Heat shock protein 40; HSP40; HSPF1; HSPF1Hdj1; Human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1